Detailed information of TA system TA065365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2186383..2187086
• Replicon: chromosome
• Accession: NZ_CP016888
• Organism: Mycobacterium tuberculosis strain SCAID 252.0

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: BEE64_RS10135
• Protein ID: WP_003409778.1
• Coordinates: 2186383..2186712 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: BEE64_RS21345
• Protein ID: WP_003409780.1
• Coordinates: 2186709..2187086 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEE64_RS10115	2182766..2183836	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
BEE64_RS10120	2183833..2184348	+	516	WP_003409718.1	flavin reductase family protein	-
BEE64_RS10125	2184345..2185406	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
BEE64_RS10130	2185403..2186173	+	771	WP_003409775.1	SDR family oxidoreductase	-
BEE64_RS10135	2186383..2186712	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BEE64_RS21345	2186709..2187086	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
BEE64_RS10145	2187083..2187673	-	591	WP_003409784.1	SEC-C domain-containing protein	-
BEE64_RS10150	2187728..2189092	+	1365	WP_068986959.1	HNH endonuclease	-
BEE64_RS10155	2189247..2189699	-	453	WP_003899095.1	lipoprotein	-
BEE64_RS10160	2189763..2190164	+	402	WP_003409869.1	hypothetical protein	-
BEE64_RS10165	2190157..2190339	-	183	WP_003409870.1	hypothetical protein	-
BEE64_RS10170	2190453..2190803	-	351	WP_003409871.1	hypothetical protein	-
BEE64_RS10175	2190814..2191716	-	903	WP_003899097.1	hypothetical protein	-
BEE64_RS10180	2191737..2191928	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T65365 WP_003409778.1 NZ_CP016888:c2186712-2186383 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T65365 NZ_CP016888:c2186712-2186383 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT65365 WP_003409780.1 NZ_CP016888:c2187086-2186709 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT65365 NZ_CP016888:c2187086-2186709 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8