Detailed information of TA system TA065363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1943673..1944286
• Replicon: chromosome
• Accession: NZ_CP016888
• Organism: Mycobacterium tuberculosis strain SCAID 252.0

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BEE64_RS09035
• Protein ID: WP_003910864.1
• Coordinates: 1943673..1944062 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ52
• Locus tag: BEE64_RS09040
• Protein ID: WP_003408469.1
• Coordinates: 1944059..1944286 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEE64_RS09000	1939302..1940216	+	915	WP_003898985.1	3-hydroxyacyl-CoA dehydrogenase family protein	-
BEE64_RS09005	1940219..1941049	+	831	WP_003408448.1	cyclase family protein	-
BEE64_RS09010	1941049..1941399	+	351	WP_003898986.1	cupin domain-containing protein	-
BEE64_RS09015	1941452..1942270	+	819	WP_003408456.1	3-keto-5-aminohexanoate cleavage protein	-
BEE64_RS09020	1942284..1943063	+	780	WP_003408460.1	IclR family transcriptional regulator	-
BEE64_RS09035	1943673..1944062	-	390	WP_003910864.1	PIN domain-containing protein	Toxin
BEE64_RS09040	1944059..1944286	-	228	WP_003408469.1	antitoxin	Antitoxin
BEE64_RS09045	1944504..1945988	+	1485	WP_003408473.1	carboxylase	-
BEE64_RS09050	1945985..1947232	+	1248	WP_003408476.1	serine hydrolase	-
BEE64_RS09055	1947275..1947694	-	420	WP_003408483.1	hypothetical protein	-
BEE64_RS09060	1947684..1948394	-	711	WP_003408486.1	winged helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (3-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 13837.02 Da
• Isoelectric Point: 7.4232

>T65363 WP_003910864.1 NZ_CP016888:c1944062-1943673 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVRAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Nucleotide

• Length: 390 bp

>T65363 NZ_CP016888:c1944062-1943673 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCGCGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8226.39 Da
• Isoelectric Point: 4.3313

>AT65363 WP_003408469.1 NZ_CP016888:c1944286-1944059 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Nucleotide

• Length: 228 bp

>AT65363 NZ_CP016888:c1944286-1944059 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4FAN9