Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1770630..1771243 Replicon chromosome
Accession NZ_CP016888
Organism Mycobacterium tuberculosis strain SCAID 252.0

Toxin (Protein)


Gene name vapC Uniprot ID P64880
Locus tag BEE64_RS08300 Protein ID WP_003407786.1
Coordinates 1770839..1771243 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8UJ46
Locus tag BEE64_RS08295 Protein ID WP_009935474.1
Coordinates 1770630..1770833 (+) Length 68 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEE64_RS08265 1766035..1766415 + 381 WP_003407768.1 fumarate reductase subunit C -
BEE64_RS08270 1766412..1766789 + 378 WP_003407771.1 fumarate reductase subunit FrdD -
BEE64_RS08275 1766857..1767465 + 609 WP_003407773.1 TetR/AcrR family transcriptional regulator -
BEE64_RS08280 1767649..1768797 + 1149 Protein_1617 MMPL family transporter -
BEE64_RS08285 1768807..1769253 + 447 WP_003407780.1 F420H(2)-dependent quinone reductase -
BEE64_RS08290 1769288..1770577 + 1290 WP_003407781.1 threonine ammonia-lyase -
BEE64_RS08295 1770630..1770833 + 204 WP_009935474.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
BEE64_RS08300 1770839..1771243 + 405 WP_003407786.1 type II toxin-antitoxin system toxin ribonuclease VapC11 Toxin
BEE64_RS08305 1771260..1773002 - 1743 WP_003407788.1 malto-oligosyltrehalose trehalohydrolase -
BEE64_RS08310 1772995..1775292 - 2298 WP_003407790.1 malto-oligosyltrehalose synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14650.76 Da        Isoelectric Point: 5.6587

>T65362 WP_003407786.1 NZ_CP016888:1770839-1771243 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Download         Length: 405 bp

>T65362 NZ_CP016888:1770839-1771243 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7501.48 Da        Isoelectric Point: 4.3951

>AT65362 WP_009935474.1 NZ_CP016888:1770630-1770833 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Download         Length: 204 bp

>AT65362 NZ_CP016888:1770630-1770833 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6A7V

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8UJ46

References