Detailed information of TA system TA065356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1238535..1239103
• Replicon: chromosome
• Accession: NZ_CP016888
• Organism: Mycobacterium tuberculosis strain SCAID 252.0

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: BEE64_RS05890
• Protein ID: WP_003405865.1
• Coordinates: 1238729..1239103 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: BEE64_RS05885
• Protein ID: WP_003405863.1
• Coordinates: 1238535..1238732 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEE64_RS05865	1234576..1235214	-	639	WP_003898730.1	lipid droplet-associated protein	-
BEE64_RS05870	1235304..1236311	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
BEE64_RS05875	1236328..1237311	-	984	WP_003898731.1	hypothetical protein	-
BEE64_RS05880	1237374..1238447	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
BEE64_RS05885	1238535..1238732	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BEE64_RS05890	1238729..1239103	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
BEE64_RS05895	1239306..1240004	+	699	WP_003898733.1	hypothetical protein	-
BEE64_RS05900	1240122..1240307	+	186	WP_003901093.1	hypothetical protein	-
BEE64_RS05905	1240234..1240509	-	276	WP_003405867.1	hypothetical protein	-
BEE64_RS05910	1240752..1241075	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
BEE64_RS05915	1241090..1241800	-	711	WP_003911399.1	hypothetical protein	-
BEE64_RS22490	1241822..1242031	+	210	WP_003911400.1	hypothetical protein	-
BEE64_RS05925	1241983..1242690	-	708	Protein_1160	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T65356 WP_003405865.1 NZ_CP016888:1238729-1239103 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T65356 NZ_CP016888:1238729-1239103 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT65356 WP_003405863.1 NZ_CP016888:1238535-1238732 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT65356 NZ_CP016888:1238535-1238732 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07