Detailed information of TA system TA065328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbE-relB/ParE-YafN
• Location: 3203711..3204206
• Replicon: chromosome
• Accession: NZ_CP016876
• Organism: Legionella pneumophila subsp. pneumophila strain FFI337

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: Q5WW71
• Locus tag: BE846_RS14425
• Protein ID: WP_011215616.1
• Coordinates: 3203949..3204206 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q5WW72
• Locus tag: BE846_RS14420
• Protein ID: WP_011215615.1
• Coordinates: 3203711..3203959 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE846_RS14390	3198771..3199388	-	618	WP_011215613.1	hypothetical protein	-
BE846_RS14395	3199400..3200569	-	1170	WP_080020610.1	AAA family ATPase	-
BE846_RS14410	3201849..3203237	-	1389	Protein_2824	MobA/MobL family protein	-
BE846_RS14420	3203711..3203959	+	249	WP_011215615.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BE846_RS14425	3203949..3204206	+	258	WP_011215616.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BE846_RS14430	3204375..3205760	+	1386	WP_011215617.1	MFS transporter	-
BE846_RS14435	3205885..3206403	-	519	WP_011215618.1	lpg1453 family Dot/Icm T4SS effector	-
BE846_RS14440	3206530..3207381	-	852	WP_041173769.1	alpha/beta hydrolase	-
BE846_RS14445	3207613..3208062	+	450	WP_011215620.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
BE846_RS14450	3208233..3208979	-	747	WP_011215621.1	ribosomal large subunit pseudouridine synthase B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-73)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10102.81 Da
• Isoelectric Point: 10.5638

>T65328 WP_011215616.1 NZ_CP016876:3203949-3204206 [Legionella pneumophila subsp. pneumophila]
MSFELGFLDEALKEWRKLDNNTRDQFKKKLAERLINPRVPASKLSGQKDRYKIKLRNIGYRLVYEVRDAELIVGMCFPTT
QKLNR

Nucleotide

• Length: 258 bp

>T65328 NZ_CP016876:3203949-3204206 [Legionella pneumophila subsp. pneumophila]
ATGAGCTTTGAGCTTGGATTTTTAGATGAGGCATTAAAAGAATGGAGAAAGCTTGACAATAATACACGCGATCAATTTAA
GAAAAAACTTGCAGAGCGTCTTATCAATCCTCGTGTACCTGCCTCGAAATTAAGTGGTCAAAAAGATCGGTATAAAATTA
AACTTCGGAACATTGGTTACCGGTTGGTCTACGAAGTTCGAGATGCTGAACTAATCGTTGGGATGTGTTTCCCGACTACT
CAAAAATTGAACAGATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9139.61 Da
• Isoelectric Point: 7.9696

>AT65328 WP_011215615.1 NZ_CP016876:3203711-3203959 [Legionella pneumophila subsp. pneumophila]
MTNVIFAQVTASISELKKNPMATVSSGDGNPVVILNRNQPAFYCIPAKLYEKFMNKLEDIELNSIADSRKNNPVIKVKLD
EL

Nucleotide

• Length: 249 bp

>AT65328 NZ_CP016876:3203711-3203959 [Legionella pneumophila subsp. pneumophila]
ATGACTAACGTTATTTTTGCACAGGTCACTGCCAGCATTTCTGAATTAAAAAAAAATCCAATGGCAACTGTTTCATCTGG
CGATGGCAATCCTGTAGTTATTTTAAATCGCAATCAACCTGCTTTTTATTGTATCCCAGCAAAGCTTTATGAAAAATTCA
TGAATAAACTTGAAGATATAGAGCTTAATAGCATTGCTGATTCAAGAAAAAATAATCCAGTTATTAAGGTAAAATTGGAT
GAGCTTTGA

Structures

Toxin

• AlphaFold DB: Q5WW71

Antitoxin

• AlphaFold DB: Q5WW72