Detailed information of TA system TA065315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbE-relB/ParE-YafN
• Location: 836714..837209
• Replicon: chromosome
• Accession: NZ_CP016870
• Organism: Legionella pneumophila subsp. pneumophila strain FFI103

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: Q5WW71
• Locus tag: BE842_RS03860
• Protein ID: WP_011215616.1
• Coordinates: 836952..837209 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q5WW72
• Locus tag: BE842_RS03855
• Protein ID: WP_011215615.1
• Coordinates: 836714..836962 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE842_RS03825	831784..832416	-	633	WP_041173768.1	hypothetical protein	-
BE842_RS03830	832413..833582	-	1170	WP_080020610.1	AAA family ATPase	-
BE842_RS03835	833627..834791	-	1165	Protein_757	IS3 family transposase	-
BE842_RS03845	834852..836240	-	1389	Protein_758	MobA/MobL family protein	-
BE842_RS03855	836714..836962	+	249	WP_011215615.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BE842_RS03860	836952..837209	+	258	WP_011215616.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BE842_RS03865	837378..838763	+	1386	WP_011215617.1	MFS transporter	-
BE842_RS03870	838888..839406	-	519	WP_011215618.1	lpg1453 family Dot/Icm T4SS effector	-
BE842_RS03875	839533..840384	-	852	WP_041173769.1	alpha/beta hydrolase	-
BE842_RS03880	840616..841065	+	450	WP_011215620.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
BE842_RS03885	841236..841982	-	747	WP_011215621.1	ribosomal large subunit pseudouridine synthase B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	aph(9)-Ia	lpg1578 / ravY / pilF / pilD / pilC / pilB / pilB / lem10 / ravX / lgt3/legC5 / lpg1484 / legK1 / lpg1453 / legL3 / lpg1661 / lpg1666 / lpg1667 / lpg1670 / mavA / fliK / lpg1689 / legC3/ppeA / ppeB / lpg1716 / ankI/legAS4 / lpg1751 / lpg1752 / fliJ / fliI / fliH / fliG / fliF / fliE / fleR/flrC / fleS/flrB	679061..1015167	336106

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-73)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10102.81 Da
• Isoelectric Point: 10.5638

>T65315 WP_011215616.1 NZ_CP016870:836952-837209 [Legionella pneumophila subsp. pneumophila]
MSFELGFLDEALKEWRKLDNNTRDQFKKKLAERLINPRVPASKLSGQKDRYKIKLRNIGYRLVYEVRDAELIVGMCFPTT
QKLNR

Nucleotide

• Length: 258 bp

>T65315 NZ_CP016870:836952-837209 [Legionella pneumophila subsp. pneumophila]
ATGAGCTTTGAGCTTGGATTTTTAGATGAGGCATTAAAAGAATGGAGAAAGCTTGACAATAATACACGCGATCAATTTAA
GAAAAAACTTGCAGAGCGTCTTATCAATCCTCGTGTACCTGCCTCGAAATTAAGTGGTCAAAAAGATCGGTATAAAATTA
AACTTCGGAACATTGGTTACCGGTTGGTCTACGAAGTTCGAGATGCTGAACTAATCGTTGGGATGTGTTTCCCGACTACT
CAAAAATTGAACAGATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9139.61 Da
• Isoelectric Point: 7.9696

>AT65315 WP_011215615.1 NZ_CP016870:836714-836962 [Legionella pneumophila subsp. pneumophila]
MTNVIFAQVTASISELKKNPMATVSSGDGNPVVILNRNQPAFYCIPAKLYEKFMNKLEDIELNSIADSRKNNPVIKVKLD
EL

Nucleotide

• Length: 249 bp

>AT65315 NZ_CP016870:836714-836962 [Legionella pneumophila subsp. pneumophila]
ATGACTAACGTTATTTTTGCACAGGTCACTGCCAGCATTTCTGAATTAAAAAAAAATCCAATGGCAACTGTTTCATCTGG
CGATGGCAATCCTGTAGTTATTTTAAATCGCAATCAACCTGCTTTTTATTGTATCCCAGCAAAGCTTTATGAAAAATTCA
TGAATAAACTTGAAGATATAGAGCTTAATAGCATTGCTGATTCAAGAAAAAATAATCCAGTTATTAAGGTAAAATTGGAT
GAGCTTTGA

Structures

Toxin

• AlphaFold DB: Q5WW71

Antitoxin

• AlphaFold DB: Q5WW72