Detailed information of TA system TA065313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbE-relB/ParE-YafN
• Location: 1141663..1142158
• Replicon: chromosome
• Accession: NZ_CP016868
• Organism: Legionella pneumophila subsp. pneumophila strain FFI102

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: Q5WW71
• Locus tag: BE841_RS04895
• Protein ID: WP_011215616.1
• Coordinates: 1141663..1141920 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q5WW72
• Locus tag: BE841_RS04900
• Protein ID: WP_011215615.1
• Coordinates: 1141910..1142158 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE841_RS04870	1136890..1137636	+	747	WP_011215621.1	ribosomal large subunit pseudouridine synthase B	-
BE841_RS04875	1137807..1138256	-	450	WP_011215620.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
BE841_RS04880	1138488..1139339	+	852	WP_041173769.1	alpha/beta hydrolase	-
BE841_RS04885	1139466..1139984	+	519	WP_011215618.1	lpg1453 family Dot/Icm T4SS effector	-
BE841_RS04890	1140109..1141494	-	1386	WP_011215617.1	MFS transporter	-
BE841_RS04895	1141663..1141920	-	258	WP_011215616.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BE841_RS04900	1141910..1142158	-	249	WP_011215615.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BE841_RS04910	1142632..1144020	+	1389	Protein_956	MobA/MobL family protein	-
BE841_RS04920	1144081..1145245	+	1165	Protein_957	IS3 family transposase	-
BE841_RS04925	1145290..1146459	+	1170	WP_080020610.1	AAA family ATPase	-
BE841_RS04930	1146456..1147088	+	633	WP_041173768.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-73)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10102.81 Da
• Isoelectric Point: 10.5638

>T65313 WP_011215616.1 NZ_CP016868:c1141920-1141663 [Legionella pneumophila subsp. pneumophila]
MSFELGFLDEALKEWRKLDNNTRDQFKKKLAERLINPRVPASKLSGQKDRYKIKLRNIGYRLVYEVRDAELIVGMCFPTT
QKLNR

Nucleotide

• Length: 258 bp

>T65313 NZ_CP016868:c1141920-1141663 [Legionella pneumophila subsp. pneumophila]
ATGAGCTTTGAGCTTGGATTTTTAGATGAGGCATTAAAAGAATGGAGAAAGCTTGACAATAATACACGCGATCAATTTAA
GAAAAAACTTGCAGAGCGTCTTATCAATCCTCGTGTACCTGCCTCGAAATTAAGTGGTCAAAAAGATCGGTATAAAATTA
AACTTCGGAACATTGGTTACCGGTTGGTCTACGAAGTTCGAGATGCTGAACTAATCGTTGGGATGTGTTTCCCGACTACT
CAAAAATTGAACAGATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9139.61 Da
• Isoelectric Point: 7.9696

>AT65313 WP_011215615.1 NZ_CP016868:c1142158-1141910 [Legionella pneumophila subsp. pneumophila]
MTNVIFAQVTASISELKKNPMATVSSGDGNPVVILNRNQPAFYCIPAKLYEKFMNKLEDIELNSIADSRKNNPVIKVKLD
EL

Nucleotide

• Length: 249 bp

>AT65313 NZ_CP016868:c1142158-1141910 [Legionella pneumophila subsp. pneumophila]
ATGACTAACGTTATTTTTGCACAGGTCACTGCCAGCATTTCTGAATTAAAAAAAAATCCAATGGCAACTGTTTCATCTGG
CGATGGCAATCCTGTAGTTATTTTAAATCGCAATCAACCTGCTTTTTATTGTATCCCAGCAAAGCTTTATGAAAAATTCA
TGAATAAACTTGAAGATATAGAGCTTAATAGCATTGCTGATTCAAGAAAAAATAATCCAGTTATTAAGGTAAAATTGGAT
GAGCTTTGA

Structures

Toxin

• AlphaFold DB: Q5WW71

Antitoxin

• AlphaFold DB: Q5WW72