Detailed information of TA system TA065312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 255623..257079
• Replicon: chromosome
• Accession: NZ_CP016868
• Organism: Legionella pneumophila subsp. pneumophila strain FFI102

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: A0A0W0UKG6
• Locus tag: BE841_RS01135
• Protein ID: WP_044496813.1
• Coordinates: 256228..257079 (+)
• Length: 284 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: BE841_RS01130
• Protein ID: WP_044496821.1
• Coordinates: 255623..256231 (+)
• Length: 203 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BE841_RS01105	250938..251897	+	960	WP_145923833.1	ATP-dependent Clp protease ATP-binding subunit	-
BE841_RS01110	251981..253144	+	1164	WP_196219907.1	MFS transporter	-
BE841_RS01115	253151..254203	-	1053	WP_044496812.1	LD-carboxypeptidase	-
BE841_RS15250	254328..254453	-	126	WP_080444601.1	IS3 family transposase	-
BE841_RS01120	254411..254803	-	393	WP_052585451.1	IS3 family transposase	-
BE841_RS15665	254812..255084	-	273	Protein_223	transposase	-
BE841_RS01130	255623..256231	+	609	WP_044496821.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
BE841_RS01135	256228..257079	+	852	WP_044496813.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
BE841_RS01140	257159..259099	-	1941	WP_058450389.1	DUF2779 domain-containing protein	-
BE841_RS01145	259342..259584	+	243	WP_058450390.1	hypothetical protein	-
BE841_RS01150	259746..260183	-	438	WP_061466918.1	conjugal transfer protein TraM	-
BE841_RS01155	260183..260908	-	726	WP_058450392.1	conjugal transfer protein TraL	-
BE841_RS01160	260908..261252	-	345	WP_058450393.1	TraK family protein	-
BE841_RS01165	261646..261999	+	354	WP_058450394.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	lpg2541 / lpg2539 / lpg2538 / lem27 / lpg2527 / mavL / sidC / sdcA / sdeD/laiF / sdjA / lpg2505 / ceg32/sidI / mavJ / lepB / sdbB / sidD / sidM/drrA / lpg2461 / ankD/legA15 / ankF/legA14/ceg31 / mavI / lpg2443 / lpg2434 / ceg30 / mavH / mavG / lem25 / lpg2420 / legA1 / lem24 / vpdA / ceg29 / lem23 / legL7 / legL6 / sdbC / katB / lpg2382 / lpg2370	43826..288712	244886

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-262)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (11-56)

Sequences

Toxin

Protein

• Length: 284 a.a.
• Molecular weight: 31759.66 Da
• Isoelectric Point: 6.2796

>T65312 WP_044496813.1 NZ_CP016868:256228-257079 [Legionella pneumophila subsp. pneumophila]
MTKNIGESVRSRLKNIAIKEGSDFNAILTRYGLERLLYRIGESEYSNQFLLKGALLFNLWYDMPHRPTKDIDLLGFGDND
LAYIKQTFSKICSISADDGINFLSESVTVDTIKKDGGYTGARVELFGELAKAKIKIQIDIGYGDAVTPGPIDAHYPVLLN
DLPAPKIRTYPIYTVIAEKLHAIALLGMANSRLKDYLDLYVLLSNEQLDNQVLAKAIQATFTRRGMAIPEELPIGLTDEF
ANDPSRESMWKAFLRKNELEQKPLTEVIAVIRNLIQVPYSLAK

Nucleotide

• Length: 852 bp

>T65312 NZ_CP016868:256228-257079 [Legionella pneumophila subsp. pneumophila]
ATGACAAAAAATATTGGTGAGTCTGTGCGCTCACGTTTGAAGAATATAGCTATAAAAGAAGGGTCTGATTTTAACGCGAT
ATTGACTCGTTACGGACTAGAACGTTTGTTATATCGGATTGGAGAATCAGAATATTCTAATCAGTTCTTGTTGAAGGGTG
CTTTACTTTTTAACCTATGGTATGACATGCCACATCGACCCACTAAAGATATTGATTTATTGGGGTTTGGTGATAATGAT
CTAGCGTATATTAAACAAACGTTTAGTAAAATCTGCAGCATCTCCGCAGATGATGGAATAAACTTTTTATCTGAATCAGT
GACGGTAGACACTATAAAAAAAGATGGTGGGTATACTGGAGCAAGAGTTGAATTATTTGGGGAATTAGCTAAAGCAAAGA
TTAAAATACAAATTGATATTGGTTATGGTGATGCAGTTACACCTGGTCCAATAGATGCCCATTATCCCGTTCTCCTTAAT
GATCTTCCTGCTCCTAAGATCCGTACTTATCCAATTTATACGGTAATCGCAGAAAAATTGCATGCTATTGCATTATTGGG
CATGGCTAATAGTCGGTTAAAAGACTACTTGGATCTTTATGTGTTATTAAGTAATGAGCAGTTAGATAATCAAGTCTTGG
CTAAAGCTATACAGGCTACATTTACTCGTCGAGGTATGGCTATTCCTGAAGAGCTGCCTATTGGTCTGACTGATGAGTTT
GCTAATGATCCAAGCAGAGAGTCGATGTGGAAAGCCTTTTTGAGAAAAAATGAACTAGAGCAGAAACCATTAACTGAAGT
TATTGCTGTGATCAGAAATTTGATTCAAGTGCCATATTCTTTGGCAAAGTAA

Antitoxin

Protein

• Length: 203 a.a.
• Molecular weight: 23043.21 Da
• Isoelectric Point: 9.7388

>AT65312 WP_044496821.1 NZ_CP016868:255623-256231 [Legionella pneumophila subsp. pneumophila]
MKTKDSYKQIVLQMARKTGVIKMSDLTKQGITRATVSRLVNENKLEKLAPGLYCLPETKFSEKESLIIIASRVPQAVFCL
LTALQIHDLTTQLPRKVWIAMPKGSHVPKMDYPPLKMVQYSDEAFSEGIEIIESDNIKLRVYKCAKTIADCFKHRNKIGL
DVAIEALKEAYTKNKVTVDELWHYAKICRVANVMRPYIEAIQ

Nucleotide

• Length: 609 bp

>AT65312 NZ_CP016868:255623-256231 [Legionella pneumophila subsp. pneumophila]
ATGAAGACAAAGGATAGCTATAAACAAATAGTACTCCAAATGGCTAGAAAAACGGGTGTAATCAAAATGTCCGATCTAAC
CAAACAGGGGATAACTAGAGCGACCGTTTCAAGACTTGTTAACGAAAACAAACTTGAAAAACTTGCTCCCGGACTATATT
GCCTACCCGAGACTAAGTTTTCTGAAAAGGAAAGCCTGATTATTATAGCAAGCCGAGTACCTCAAGCCGTGTTTTGTCTG
CTAACTGCTTTGCAGATCCATGATCTAACAACACAACTACCTCGAAAAGTATGGATCGCTATGCCAAAGGGAAGTCATGT
GCCAAAAATGGATTATCCGCCGCTAAAAATGGTGCAATATTCAGATGAAGCCTTTAGTGAAGGAATTGAAATTATAGAAT
CAGATAATATTAAACTAAGGGTGTATAAATGTGCCAAGACAATTGCAGATTGTTTCAAACATCGCAATAAAATTGGACTT
GATGTGGCCATTGAAGCGCTCAAAGAGGCTTATACAAAAAATAAAGTAACAGTTGATGAACTATGGCATTATGCGAAAAT
TTGTCGCGTTGCAAACGTAATGCGTCCTTATATTGAGGCAATTCAATGA

Structures

Toxin

• AlphaFold DB: A0A0W0UKG6