Detailed information of TA system TA065267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2064739..2065268
• Replicon: chromosome
• Accession: NZ_CP016858
• Organism: Staphylococcus aureus strain 1971.C01

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: A7U47_RS10910
• Protein ID: WP_000621175.1
• Coordinates: 2064906..2065268 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: A7U47_RS10905
• Protein ID: WP_000948331.1
• Coordinates: 2064739..2064909 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7U47_RS10875	2059776..2060336	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
A7U47_RS10880	2060544..2061023	+	480	WP_001287088.1	hypothetical protein	-
A7U47_RS10885	2061016..2062599	+	1584	WP_001294626.1	PH domain-containing protein	-
A7U47_RS10890	2062586..2063077	+	492	WP_001205910.1	PH domain-containing protein	-
A7U47_RS10895	2063081..2063440	+	360	WP_000581200.1	holo-ACP synthase	-
A7U47_RS10900	2063506..2064654	+	1149	WP_001281145.1	alanine racemase	-
A7U47_RS10905	2064739..2064909	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
A7U47_RS10910	2064906..2065268	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A7U47_RS10920	2065618..2066619	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
A7U47_RS10925	2066738..2067064	+	327	WP_001052491.1	anti-sigma factor antagonist	-
A7U47_RS10930	2067066..2067545	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
A7U47_RS10935	2067520..2068290	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T65267 WP_000621175.1 NZ_CP016858:2064906-2065268 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T65267 NZ_CP016858:2064906-2065268 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT65267 WP_000948331.1 NZ_CP016858:2064739-2064909 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT65267 NZ_CP016858:2064739-2064909 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1