Detailed information of TA system TA065250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2050841..2051370
• Replicon: chromosome
• Accession: NZ_CP016856
• Organism: Staphylococcus aureus strain 2148.N

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: A7U44_RS10420
• Protein ID: WP_000621175.1
• Coordinates: 2051008..2051370 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: A7U44_RS10415
• Protein ID: WP_000948331.1
• Coordinates: 2050841..2051011 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7U44_RS10385	2045877..2046437	+	561	WP_001092402.1	K(+)-transporting ATPase subunit C	-
A7U44_RS10390	2046646..2047125	+	480	WP_001287090.1	hypothetical protein	-
A7U44_RS10395	2047118..2048701	+	1584	WP_001294626.1	PH domain-containing protein	-
A7U44_RS10400	2048688..2049179	+	492	WP_001205910.1	PH domain-containing protein	-
A7U44_RS10405	2049183..2049542	+	360	WP_000581197.1	holo-ACP synthase	-
A7U44_RS10410	2049608..2050756	+	1149	WP_001281142.1	alanine racemase	-
A7U44_RS10415	2050841..2051011	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
A7U44_RS10420	2051008..2051370	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A7U44_RS10430	2051719..2052720	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
A7U44_RS10435	2052839..2053165	+	327	WP_001052491.1	anti-sigma factor antagonist	-
A7U44_RS10440	2053167..2053646	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
A7U44_RS10445	2053621..2054391	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T65250 WP_000621175.1 NZ_CP016856:2051008-2051370 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T65250 NZ_CP016856:2051008-2051370 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT65250 WP_000948331.1 NZ_CP016856:2050841-2051011 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT65250 NZ_CP016856:2050841-2051011 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1