Detailed information of TA system TA065249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2031115..2031629
• Replicon: chromosome
• Accession: NZ_CP016856
• Organism: Staphylococcus aureus strain 2148.N

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: A7U44_RS10330
• Protein ID: WP_000581792.1
• Coordinates: 2031420..2031629 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: A7U44_RS10325
• Protein ID: WP_000138932.1
• Coordinates: 2031115..2031408 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7U44_RS10300	2026719..2027360	+	642	WP_000483153.1	thiamine phosphate synthase	-
A7U44_RS10305	2027446..2028318	+	873	WP_000725802.1	membrane protein insertase YidC	-
A7U44_RS10315	2028790..2029437	-	648	WP_001187630.1	HD domain-containing protein	-
A7U44_RS10320	2029465..2030949	-	1485	WP_000571549.1	cardiolipin synthase	-
A7U44_RS10325	2031115..2031408	+	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
A7U44_RS10330	2031420..2031629	+	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
A7U44_RS10335	2031757..2031894	+	138	WP_000828354.1	hypothetical protein	-
A7U44_RS10340	2032158..2033360	-	1203	WP_001109939.1	rod shape-determining protein RodA	-
A7U44_RS10345	2033678..2034748	+	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
A7U44_RS10350	2034763..2036121	+	1359	WP_000611470.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T65249 WP_000581792.1 NZ_CP016856:2031420-2031629 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T65249 NZ_CP016856:2031420-2031629 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT65249 WP_000138932.1 NZ_CP016856:2031115-2031408 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT65249 NZ_CP016856:2031115-2031408 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13