Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1347889..1348805 Replicon chromosome
Accession NZ_CP016852
Organism Bacillus subtilis subsp. subtilis strain 168G

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag BFI33_RS07150 Protein ID WP_003244695.1
Coordinates 1348059..1348805 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag BFI33_RS07145 Protein ID WP_003232646.1
Coordinates 1347889..1348059 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BFI33_RS07115 1344752..1345081 + 330 WP_003232660.1 phage-like element PBSX protein XkdW -
BFI33_RS07120 1345078..1345242 + 165 WP_003232658.1 XkdX family protein -
BFI33_RS07125 1345286..1346125 + 840 WP_003245597.1 phage-like element PBSX protein XepA -
BFI33_RS07130 1346178..1346447 + 270 WP_003232655.1 hemolysin XhlA family protein -
BFI33_RS07135 1346460..1346723 + 264 WP_003232653.1 phage holin -
BFI33_RS07140 1346736..1347629 + 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
BFI33_RS22745 1347666..1347803 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
BFI33_RS07145 1347889..1348059 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
BFI33_RS07150 1348059..1348805 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
BFI33_RS07155 1348915..1349916 - 1002 WP_003232642.1 inorganic phosphate transporter -
BFI33_RS07160 1349929..1350546 - 618 WP_003218470.1 DUF47 domain-containing protein -
BFI33_RS07165 1350822..1352138 - 1317 WP_003244819.1 serine/threonine exchanger -
BFI33_RS07170 1352527..1353477 + 951 WP_003245086.1 ring-cleaving dioxygenase -
BFI33_RS22750 1353578..1353724 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T65200 WP_003244695.1 NZ_CP016852:c1348805-1348059 [Bacillus subtilis subsp. subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T65200 NZ_CP016852:c1348805-1348059 [Bacillus subtilis subsp. subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT65200 WP_003232646.1 NZ_CP016852:c1348059-1347889 [Bacillus subtilis subsp. subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT65200 NZ_CP016852:c1348059-1347889 [Bacillus subtilis subsp. subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References