Detailed information of TA system TA065198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE-Phd
• Location: 38206..38833
• Replicon: plasmid pL21634-2
• Accession: NZ_CP016851
• Organism: Pseudomonas sp. TMW 2.1634

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: BFC21_RS26955
• Protein ID: WP_094991502.1
• Coordinates: 38206..38571 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: -
• Locus tag: BFC21_RS26960
• Protein ID: WP_120266843.1
• Coordinates: 38585..38833 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFC21_RS26915	33268..33687	-	420	WP_120266824.1	hypothetical protein	-
BFC21_RS27400	33697..34110	-	414	WP_162939420.1	hypothetical protein	-
BFC21_RS26920	34116..34742	-	627	WP_120266825.1	recombinase family protein	-
BFC21_RS26925	35015..35284	-	270	WP_120266826.1	hypothetical protein	-
BFC21_RS26930	35371..35790	-	420	WP_120266827.1	hypothetical protein	-
BFC21_RS26940	36565..36891	-	327	WP_120266828.1	hypothetical protein	-
BFC21_RS26945	36979..37260	-	282	WP_153351099.1	hypothetical protein	-
BFC21_RS26950	37473..37769	+	297	WP_120266830.1	hypothetical protein	-
BFC21_RS26955	38206..38571	-	366	WP_094991502.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFC21_RS26960	38585..38833	-	249	WP_120266843.1	prevent-host-death protein	Antitoxin
BFC21_RS26965	39317..42283	-	2967	WP_070414742.1	Tn3-like element TnAs1 family transposase	-
BFC21_RS26970	42287..42847	-	561	WP_001161490.1	recombinase family protein	-
BFC21_RS26975	43145..43621	+	477	WP_011711661.1	DM13 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56859	56859

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (10-99)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13640.52 Da
• Isoelectric Point: 4.4787

>T65198 WP_094991502.1 NZ_CP016851:c38571-38206 [Pseudomonas sp. TMW 2.1634]
MTSLSDSYTVDFTHTAELSVEDQIEHLAIYQGTDSALTRVLGTIDVAAQKLATNPKGYKVCPQASELGVLNYRELNTDRF
RFIYEIMEDELRVVVHLVLGEKQSVEDALVRNCLLLPLRPE

Nucleotide

• Length: 366 bp

>T65198 NZ_CP016851:c38571-38206 [Pseudomonas sp. TMW 2.1634]
ATGACGTCACTATCGGACAGTTACACAGTAGATTTCACCCACACCGCTGAACTAAGCGTCGAAGACCAGATTGAACATCT
GGCCATCTATCAAGGCACCGACTCTGCGCTTACACGCGTACTCGGTACGATTGACGTTGCTGCGCAAAAGCTCGCAACCA
ATCCGAAGGGCTATAAAGTTTGCCCTCAAGCCAGTGAATTAGGTGTCCTGAATTACCGTGAGCTCAACACTGACAGGTTT
CGGTTCATCTACGAAATCATGGAAGACGAGCTGCGTGTTGTTGTTCACCTGGTACTGGGTGAGAAACAGAGCGTTGAAGA
TGCGCTTGTCCGTAACTGTCTGCTGCTACCACTTCGCCCTGAGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9258.54 Da
• Isoelectric Point: 8.9207

>AT65198 WP_120266843.1 NZ_CP016851:c38833-38585 [Pseudomonas sp. TMW 2.1634]
VRVETVSFLKKYASDLPLDEPMVVTQNGVPAYVVESYAERKRRDEAIALVKLLAISSRDYAQGNHCSTDELKARLSRRFA
NT

Nucleotide

• Length: 249 bp

>AT65198 NZ_CP016851:c38833-38585 [Pseudomonas sp. TMW 2.1634]
GTGAGAGTTGAAACAGTTAGTTTTTTGAAAAAATACGCATCCGACCTGCCTCTAGATGAGCCCATGGTTGTCACGCAAAA
CGGTGTACCTGCATACGTTGTGGAGTCTTACGCAGAGCGTAAACGTCGCGATGAAGCAATTGCCCTTGTGAAATTACTCG
CAATCAGTTCCCGCGATTATGCCCAAGGCAACCACTGTTCGACTGATGAACTGAAAGCACGCCTGAGCAGGAGGTTTGCC
AACACATAA