Detailed information of TA system TA065197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 53123..53670
• Replicon: plasmid pL21564-1
• Accession: NZ_CP016850
• Organism: Pseudomonas sp. TMW 2.1634

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S6AKK9
• Locus tag: BFC21_RS26535
• Protein ID: WP_016502317.1
• Coordinates: 53123..53398 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S6AY38
• Locus tag: BFC21_RS26540
• Protein ID: WP_016502318.1
• Coordinates: 53398..53670 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFC21_RS26515	48424..49839	-	1416	WP_019334892.1	glycosyltransferase family 39 protein	-
BFC21_RS26520	49839..50825	-	987	WP_100226690.1	glycosyltransferase family 2 protein	-
BFC21_RS26525	50806..51201	-	396	WP_080034397.1	GtrA family protein	-
BFC21_RS26530	51577..52848	-	1272	WP_023082049.1	IS4-like element ISPa1635 family transposase	-
BFC21_RS26535	53123..53398	-	276	WP_016502317.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFC21_RS26540	53398..53670	-	273	WP_016502318.1	ribbon-helix-helix domain-containing protein	Antitoxin
BFC21_RS26545	53684..54097	-	414	WP_033901201.1	hypothetical protein	-
BFC21_RS26550	54104..54844	-	741	WP_019334897.1	DUF2807 domain-containing protein	-
BFC21_RS26555	55075..55638	+	564	WP_019334898.1	recombinase family protein	-
BFC21_RS26560	55592..56485	-	894	WP_120266775.1	hypothetical protein	-
BFC21_RS26565	56491..56952	-	462	WP_120266776.1	conjugal transfer protein TrbH	-
BFC21_RS26570	56952..57848	-	897	WP_086799791.1	P-type conjugative transfer protein TrbG	-
BFC21_RS26575	57869..58600	-	732	WP_120266777.1	conjugal transfer protein TrbF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	kdsA	1..99718	99718
	-	inside	IScluster/Tn	-	-	44478..55638	11160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-47)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10702.22 Da
• Isoelectric Point: 7.2020

>T65197 WP_016502317.1 NZ_CP016850:c53398-53123 [Pseudomonas sp. TMW 2.1634]
MELKWTSKALSDIARLYEFLASVNQPAAARTVQQLTAAPSTLLANPRIGERLEEFDPRDVRRIQIGHYEMRYEIVDSTIY
LLRLWHTREDR

Nucleotide

• Length: 276 bp

>T65197 NZ_CP016850:c53398-53123 [Pseudomonas sp. TMW 2.1634]
ATGGAGTTGAAGTGGACAAGCAAGGCGCTTTCCGACATTGCCCGGTTGTATGAGTTTCTGGCGTCGGTGAATCAGCCTGC
CGCCGCACGAACAGTGCAGCAACTCACAGCCGCACCTTCCACGCTGCTGGCCAACCCGCGCATTGGCGAACGCCTGGAAG
AATTCGATCCACGGGATGTGCGCCGAATTCAGATCGGCCATTACGAGATGCGTTACGAGATAGTGGATTCCACTATCTAC
CTACTGCGCCTGTGGCACACCCGCGAAGACCGATAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10074.43 Da
• Isoelectric Point: 5.7066

>AT65197 WP_016502318.1 NZ_CP016850:c53670-53398 [Pseudomonas sp. TMW 2.1634]
MATTAKTRSVTAHVPVQLAEKVDLMAERLERSKNWIVKQALAAWIDQEEERSRLTREALADVDAGRVIDHQAVQAWAESL
STDTPLPVPR

Nucleotide

• Length: 273 bp

>AT65197 NZ_CP016850:c53670-53398 [Pseudomonas sp. TMW 2.1634]
ATGGCAACCACCGCGAAAACCCGATCCGTCACGGCTCACGTCCCTGTGCAACTGGCCGAAAAGGTCGATTTGATGGCTGA
GCGCCTGGAACGCTCCAAGAACTGGATCGTCAAGCAGGCTCTGGCCGCCTGGATAGACCAGGAAGAAGAACGTAGTCGCC
TGACCCGTGAGGCATTGGCCGATGTGGATGCTGGTCGCGTGATCGATCATCAAGCTGTCCAGGCCTGGGCTGAAAGCCTC
AGCACTGACACTCCGTTGCCGGTACCGCGCTGA

Structures

Toxin

• AlphaFold DB: S6AKK9

Antitoxin

• AlphaFold DB: S6AY38