Detailed information of TA system TA065193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3661951..3662555
• Replicon: chromosome
• Accession: NZ_CP016849
• Organism: Pseudomonas sp. TMW 2.1634

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BFC21_RS17275
• Protein ID: WP_081481088.1
• Coordinates: 3662358..3662555 (-)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A494R758
• Locus tag: BFC21_RS17270
• Protein ID: WP_016783077.1
• Coordinates: 3661951..3662361 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFC21_RS17250	3657695..3659332	-	1638	WP_088378483.1	BCCT family transporter	-
BFC21_RS17255	3659630..3660712	-	1083	WP_088378484.1	tartrate dehydrogenase	-
BFC21_RS17260	3660806..3661714	+	909	WP_162939400.1	LysR family transcriptional regulator	-
BFC21_RS17270	3661951..3662361	-	411	WP_016783077.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BFC21_RS17275	3662358..3662555	-	198	WP_081481088.1	type II toxin-antitoxin system HicA family toxin	Toxin
BFC21_RS17280	3662798..3663298	+	501	WP_120266392.1	sigma-70 family RNA polymerase sigma factor	-
BFC21_RS17285	3663298..3664245	+	948	WP_120266393.1	FecR domain-containing protein	-
BFC21_RS17290	3664358..3666796	+	2439	WP_088378487.1	TonB-dependent siderophore receptor	-
BFC21_RS17295	3666894..3667301	-	408	WP_095004377.1	type II toxin-antitoxin system HicB family antitoxin	-
BFC21_RS17300	3667342..3667521	-	180	WP_095004378.1	type II toxin-antitoxin system HicA family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7226.44 Da
• Isoelectric Point: 11.0468

>T65193 WP_081481088.1 NZ_CP016849:c3662555-3662358 [Pseudomonas sp. TMW 2.1634]
MRSRELIELLMADGWFEIAVKGSHHQFKHPAKAGRVTVPHPRTEIAKGTLHSILKSAGLKGELRS

Nucleotide

• Length: 198 bp

>T65193 NZ_CP016849:c3662555-3662358 [Pseudomonas sp. TMW 2.1634]
ATGCGAAGTCGTGAACTTATCGAGTTGCTGATGGCGGATGGGTGGTTTGAGATCGCGGTTAAAGGAAGTCACCATCAGTT
CAAGCACCCAGCCAAAGCGGGTCGGGTAACGGTTCCGCATCCCAGGACAGAAATTGCCAAAGGGACGCTGCACAGCATTC
TCAAATCCGCAGGTCTCAAAGGAGAGTTACGGTCATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14844.63 Da
• Isoelectric Point: 4.4720

>AT65193 WP_016783077.1 NZ_CP016849:c3662361-3661951 [Pseudomonas sp. TMW 2.1634]
MKFPVVIHKDADSEFSVTVPDVPGCFSAGATFSQALDNVQEALALHLEGIVADGGELPQGKDVDAHLENPDYVGGIWAIV
DFDLTPYLGKSVRFNASLPENLLQRIDERVSRDHRYASRSGFLATAALRELSVQNF

Nucleotide

• Length: 411 bp

>AT65193 NZ_CP016849:c3662361-3661951 [Pseudomonas sp. TMW 2.1634]
ATGAAATTTCCAGTGGTAATACATAAAGACGCAGATTCTGAATTCAGCGTGACGGTGCCTGATGTGCCGGGCTGTTTTTC
TGCAGGGGCCACCTTTTCGCAAGCGCTGGATAACGTGCAGGAAGCATTGGCTTTGCATCTGGAAGGCATTGTTGCGGACG
GCGGTGAGCTACCCCAGGGCAAGGACGTGGACGCCCATCTGGAGAATCCGGATTATGTCGGCGGGATATGGGCAATCGTT
GACTTTGATTTGACGCCTTATCTCGGCAAGTCGGTTCGGTTCAATGCGTCTTTGCCAGAGAACCTGTTGCAGCGTATTGA
TGAACGAGTCAGCAGGGACCACCGCTATGCGTCCCGTTCGGGGTTTCTGGCCACAGCGGCATTGCGCGAGCTGAGCGTTC
AGAACTTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A494R758