Detailed information of TA system TA065192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 2840542..2841128
• Replicon: chromosome
• Accession: NZ_CP016849
• Organism: Pseudomonas sp. TMW 2.1634

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BFC21_RS13515
• Protein ID: WP_095035893.1
• Coordinates: 2840542..2840850 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: BFC21_RS13520
• Protein ID: WP_120266251.1
• Coordinates: 2840847..2841128 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BFC21_RS13490	2836094..2836810	-	717	WP_120266246.1	hypothetical protein	-
BFC21_RS13495	2836807..2838273	-	1467	WP_162939346.1	hypothetical protein	-
BFC21_RS13500	2838408..2839124	+	717	WP_120266248.1	hypothetical protein	-
BFC21_RS13505	2839285..2839992	+	708	WP_120266249.1	hypothetical protein	-
BFC21_RS13510	2840137..2840484	+	348	WP_120266250.1	hypothetical protein	-
BFC21_RS13515	2840542..2840850	+	309	WP_095035893.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BFC21_RS13520	2840847..2841128	+	282	WP_120266251.1	putative addiction module antidote protein	Antitoxin
BFC21_RS13525	2841163..2841528	-	366	WP_153404189.1	hypothetical protein	-
BFC21_RS13530	2841682..2842137	-	456	WP_120266253.1	hypothetical protein	-
BFC21_RS13535	2842377..2842979	-	603	WP_120266254.1	hypothetical protein	-
BFC21_RS13540	2843075..2843506	-	432	WP_095035888.1	hypothetical protein	-
BFC21_RS27285	2843640..2844002	-	363	WP_141232571.1	hypothetical protein	-
BFC21_RS13545	2844062..2845075	-	1014	WP_095035887.1	DNA adenine methylase	-
BFC21_RS13550	2845298..2845660	-	363	WP_095035886.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2790882..2867466	76584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-89)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11636.41 Da
• Isoelectric Point: 9.0591

>T65192 WP_095035893.1 NZ_CP016849:2840542-2840850 [Pseudomonas sp. TMW 2.1634]
MNTLIRTHEFDAWLKGLKDVQARLRIISRIMSAQEGNFGDCEPVGEGISEMRIFVGPGYRVYYTRRGEVVYLLLCGGNKS
SQKRDIKLAKSILDEIEQGDRK

Nucleotide

• Length: 309 bp

>T65192 NZ_CP016849:2840542-2840850 [Pseudomonas sp. TMW 2.1634]
ATGAACACACTCATCAGAACTCACGAATTTGACGCATGGCTGAAAGGTCTTAAAGACGTACAGGCACGTCTGAGAATCAT
CAGCCGTATCATGTCAGCACAGGAAGGGAATTTCGGAGACTGTGAGCCGGTAGGAGAAGGCATTTCAGAGATGCGGATCT
TCGTCGGACCCGGCTACCGCGTCTACTACACAAGGCGCGGTGAAGTGGTCTATCTGCTACTGTGTGGAGGCAACAAGTCA
TCACAGAAACGTGACATTAAGCTTGCGAAGTCTATCCTCGATGAGATCGAGCAAGGAGATAGAAAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10271.83 Da
• Isoelectric Point: 7.0109

>AT65192 WP_120266251.1 NZ_CP016849:2840847-2841128 [Pseudomonas sp. TMW 2.1634]
MRELKVFEASDYLNNEGEIAQFLTISLEDPNPDVFLRALQEVAKARGMSQLAKDAGINRESLYKALAPGAKPRYDTVLKL
VNALGVKLTAQHV

Nucleotide

• Length: 282 bp

>AT65192 NZ_CP016849:2840847-2841128 [Pseudomonas sp. TMW 2.1634]
ATGAGAGAACTCAAAGTCTTTGAAGCATCTGACTACCTGAACAACGAGGGCGAGATTGCTCAATTTTTGACCATTTCGTT
GGAAGACCCAAACCCTGACGTTTTCCTTCGTGCGCTGCAAGAAGTCGCCAAGGCGCGTGGAATGAGTCAGCTTGCGAAGG
ATGCAGGTATCAACCGCGAAAGTCTCTATAAGGCGCTGGCACCGGGCGCAAAACCCCGCTATGACACGGTATTGAAGTTG
GTGAATGCTTTGGGTGTGAAATTGACTGCCCAGCACGTATAA