Detailed information of TA system TA065179

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4753956..4754757
• Replicon: chromosome
• Accession: NZ_CP016828
• Organism: Escherichia coli strain FORC_043

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: D3GUT5
• Locus tag: FORC43_RS25145
• Protein ID: WP_001094430.1
• Coordinates: 4754380..4754757 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: FORC43_RS25140
• Protein ID: WP_088888735.1
• Coordinates: 4753956..4754333 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC43_RS25105	4749159..4750667	-	1509	WP_001189118.1	group II intron reverse transcriptase/maturase	-
FORC43_RS25115	4751340..4752079	-	740	Protein_4577	IS3 family transposase	-
FORC43_RS28850	4752136..4752261	+	126	Protein_4578	DUF945 domain-containing protein	-
FORC43_RS25125	4752603..4753076	+	474	WP_000855057.1	antirestriction protein	-
FORC43_RS25130	4753092..4753568	+	477	WP_157787305.1	RadC family protein	-
FORC43_RS25135	4753655..4753876	+	222	WP_000692352.1	DUF987 domain-containing protein	-
FORC43_RS25140	4753956..4754333	+	378	WP_088888735.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
FORC43_RS25145	4754380..4754757	+	378	WP_001094430.1	TA system toxin CbtA family protein	Toxin
FORC43_RS25150	4754754..4755242	+	489	WP_000761715.1	hypothetical protein	-
FORC43_RS25155	4755262..4755459	+	198	WP_000772029.1	DUF957 domain-containing protein	-
FORC43_RS25160	4755544..4756386	+	843	WP_001290187.1	DUF4942 domain-containing protein	-
FORC43_RS25170	4757135..4758673	+	1539	WP_001187182.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13971.82 Da
• Isoelectric Point: 6.8608

>T65179 WP_001094430.1 NZ_CP016828:4754380-4754757 [Escherichia coli]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T65179 NZ_CP016828:4754380-4754757 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGAATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13705.60 Da
• Isoelectric Point: 6.0568

>AT65179 WP_088888735.1 NZ_CP016828:4753956-4754333 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Nucleotide

• Length: 378 bp

>AT65179 NZ_CP016828:4753956-4754333 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACTGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y6M7