Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3931462..3932080 Replicon chromosome
Accession NZ_CP016828
Organism Escherichia coli strain FORC_043

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FORC43_RS20940 Protein ID WP_001291435.1
Coordinates 3931862..3932080 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FORC43_RS20935 Protein ID WP_000344800.1
Coordinates 3931462..3931836 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC43_RS20925 3926551..3927744 + 1194 WP_001386618.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
FORC43_RS20930 3927767..3930916 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
FORC43_RS20935 3931462..3931836 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FORC43_RS20940 3931862..3932080 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FORC43_RS20945 3932251..3932802 + 552 WP_000102564.1 maltose O-acetyltransferase -
FORC43_RS20950 3932918..3933388 + 471 WP_000136192.1 YlaC family protein -
FORC43_RS20955 3933552..3935102 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FORC43_RS20960 3935144..3935497 - 354 WP_000878140.1 DUF1428 domain-containing protein -
FORC43_RS20970 3935876..3936187 + 312 WP_000409911.1 MGMT family protein -
FORC43_RS20975 3936218..3936790 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T65175 WP_001291435.1 NZ_CP016828:3931862-3932080 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T65175 NZ_CP016828:3931862-3932080 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT65175 WP_000344800.1 NZ_CP016828:3931462-3931836 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT65175 NZ_CP016828:3931462-3931836 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References