Detailed information of TA system TA065174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3291729..3292210
• Replicon: chromosome
• Accession: NZ_CP016828
• Organism: Escherichia coli strain FORC_043

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: -
• Locus tag: FORC43_RS17535
• Protein ID: WP_033812095.1
• Coordinates: 3291920..3292210 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: -
• Locus tag: FORC43_RS17530
• Protein ID: WP_033812094.1
• Coordinates: 3291729..3291920 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC43_RS17480	3286874..3287200	-	327	WP_001369935.1	hypothetical protein	-
FORC43_RS17485	3287201..3287512	-	312	WP_001289674.1	hypothetical protein	-
FORC43_RS17490	3287499..3287804	-	306	WP_001376361.1	DUF4406 domain-containing protein	-
FORC43_RS17495	3287801..3288082	-	282	WP_087614068.1	DNA-binding protein	-
FORC43_RS17500	3288115..3288831	-	717	WP_088888717.1	DUF1627 domain-containing protein	-
FORC43_RS28405	3288865..3289407	-	543	WP_072130322.1	replication protein	-
FORC43_RS28410	3289319..3290350	-	1032	WP_157787303.1	phage replisome organizer	-
FORC43_RS17515	3290419..3290844	-	426	WP_000693925.1	Rha family transcriptional regulator	-
FORC43_RS17520	3290828..3291103	-	276	WP_047083516.1	helix-turn-helix domain-containing protein	-
FORC43_RS17525	3291211..3291711	+	501	WP_033812093.1	helix-turn-helix transcriptional regulator	-
FORC43_RS17530	3291729..3291920	+	192	WP_033812094.1	hypothetical protein	Antitoxin
FORC43_RS17535	3291920..3292210	+	291	WP_033812095.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FORC43_RS17540	3292479..3292631	+	153	WP_000380319.1	DUF1391 family protein	-
FORC43_RS28415	3292787..3293044	+	258	WP_077637644.1	hypothetical protein	-
FORC43_RS17550	3293125..3293310	-	186	WP_024213805.1	hypothetical protein	-
FORC43_RS17560	3293855..3294043	+	189	WP_000450222.1	cell division inhibitor	-
FORC43_RS17565	3294040..3294228	+	189	WP_086163675.1	DUF1482 family protein	-
FORC43_RS17570	3294321..3296723	+	2403	WP_088888718.1	exonuclease	-
FORC43_RS17575	3296790..3297041	+	252	WP_000273163.1	DUF4224 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11192.05 Da
• Isoelectric Point: 9.9854

>T65174 WP_033812095.1 NZ_CP016828:3291920-3292210 [Escherichia coli]
MLPILWLPSARDDLRQIVAYIAKENPPAARRLKIRIETSVLSLTEHPYLYPPSERVPSLREIVTHPNYIILYRVTTSNIE
IVNVVHSRRQYPNKSC

Nucleotide

• Length: 291 bp

>T65174 NZ_CP016828:3291920-3292210 [Escherichia coli]
ATGCTGCCCATTTTATGGCTACCTTCTGCACGTGATGATTTACGTCAGATAGTAGCCTATATTGCTAAAGAAAACCCTCC
CGCTGCACGTAGACTAAAAATACGCATTGAAACATCAGTTTTGTCACTTACTGAACACCCTTATCTGTACCCACCGAGCG
AAAGAGTTCCAAGTCTTCGAGAGATAGTGACTCATCCTAACTACATAATACTTTACCGAGTAACAACATCTAACATCGAG
ATCGTAAATGTAGTTCACTCACGAAGGCAGTATCCAAACAAAAGCTGTTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 6993.76 Da
• Isoelectric Point: 4.0270

>AT65174 WP_033812094.1 NZ_CP016828:3291729-3291920 [Escherichia coli]
MSTALSPIISEFETVEQENSYNEWLRAKVAASLADPRSGIPHDEVMAEMENLIAQLATTNRSE

Nucleotide

• Length: 192 bp

>AT65174 NZ_CP016828:3291729-3291920 [Escherichia coli]
ATGAGTACAGCCCTTTCCCCGATAATTTCTGAATTTGAAACGGTCGAACAAGAAAACAGCTATAACGAATGGTTGCGAGC
CAAAGTGGCAGCAAGCCTCGCAGATCCCCGTTCTGGAATTCCGCATGATGAAGTAATGGCTGAAATGGAAAACCTTATTG
CTCAATTAGCTACAACGAACAGGAGTGAGTAA