Detailed information of TA system TA065153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1175103..1175667
• Replicon: chromosome
• Accession: NZ_CP016818
• Organism: Mycobacterium avium subsp. hominissuis strain HP17

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A2A2ZLT9
• Locus tag: BEP52_RS05890
• Protein ID: WP_023868517.1
• Coordinates: 1175296..1175667 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BEP52_RS05885
• Protein ID: WP_011724021.1
• Coordinates: 1175103..1175309 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEP52_RS05865	1170357..1171355	+	999	WP_023868514.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
BEP52_RS05870	1171379..1172587	-	1209	WP_179145049.1	hypothetical protein	-
BEP52_RS05875	1172718..1173791	+	1074	WP_044543554.1	redox-regulated ATPase YchF	-
BEP52_RS05880	1173991..1174935	+	945	WP_084250736.1	HNH endonuclease	-
BEP52_RS05885	1175103..1175309	+	207	WP_011724021.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BEP52_RS05890	1175296..1175667	+	372	WP_023868517.1	type II toxin-antitoxin system VapC family toxin	Toxin
BEP52_RS05895	1175767..1176207	+	441	WP_003875392.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
BEP52_RS05900	1176273..1177010	-	738	WP_044543556.1	SDR family oxidoreductase	-
BEP52_RS05905	1177085..1177690	+	606	WP_095975041.1	TetR/AcrR family transcriptional regulator; helix-turn-helix transcriptional regulator	-
BEP52_RS05910	1177792..1178190	+	399	WP_044543557.1	VOC family protein	-
BEP52_RS05915	1178259..1178642	+	384	WP_011724026.1	hypothetical protein	-
BEP52_RS05920	1178712..1179065	+	354	WP_023865350.1	hypothetical protein	-
BEP52_RS05925	1179222..1179830	+	609	WP_080772802.1	glycoside hydrolase	-
BEP52_RS05930	1180196..1180519	+	324	WP_044543559.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13754.08 Da
• Isoelectric Point: 6.6032

>T65153 WP_023868517.1 NZ_CP016818:1175296-1175667 [Mycobacterium avium subsp. hominissuis]
MILVDTSVWIDHLHVADKRLIEFLTNDAIGCHQMVIEELALGAIRRRADLLRLLSNLRAFPLLTHAEVLHLVESHRLWGR
GLSAIDVHLLGSVALVDGARLWTRDKNLKAAGWDVGIAIVDEL

Nucleotide

• Length: 372 bp

>T65153 NZ_CP016818:1175296-1175667 [Mycobacterium avium subsp. hominissuis]
ATGATTCTCGTTGACACGTCGGTATGGATCGATCATCTGCACGTCGCCGACAAGCGACTCATAGAGTTCCTGACCAACGA
CGCAATCGGTTGCCACCAAATGGTGATCGAGGAGTTGGCATTGGGTGCGATCAGGCGGCGCGCCGATCTATTGCGCCTGC
TGTCCAATCTTCGGGCGTTCCCGTTGCTGACGCATGCGGAGGTCCTTCATCTCGTCGAAAGCCACCGCTTGTGGGGCCGC
GGACTGAGTGCGATCGACGTCCATCTTCTTGGGTCGGTTGCTCTGGTCGACGGTGCACGGCTATGGACCAGGGACAAGAA
TTTAAAAGCGGCGGGCTGGGACGTGGGTATCGCGATCGTCGACGAGCTCTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7261.25 Da
• Isoelectric Point: 11.0634

>AT65153 WP_011724021.1 NZ_CP016818:1175103-1175309 [Mycobacterium avium subsp. hominissuis]
MRTTVTIDDALLAEAAELTGVTESVALLRQGLQTLVRVESARRLAALGGTDRKASVGPRRRTPADDSR

Nucleotide

• Length: 207 bp

>AT65153 NZ_CP016818:1175103-1175309 [Mycobacterium avium subsp. hominissuis]
ATGAGGACGACGGTGACGATTGACGACGCCTTATTGGCAGAGGCGGCCGAACTGACCGGGGTGACAGAGAGCGTGGCGCT
CCTCCGTCAGGGACTGCAGACACTCGTTCGGGTAGAGAGCGCCCGTCGGCTGGCGGCGCTCGGCGGCACGGATCGGAAGG
CATCGGTCGGCCCTCGCCGGAGGACACCCGCCGATGATTCTCGTTGA

Structures

Toxin

• AlphaFold DB: A0A2A2ZLT9