Detailed information of TA system TA065151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 190958..191628
• Replicon: plasmid unamed
• Accession: NZ_CP016815
• Organism: Klebsiella pneumoniae strain ED23

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: BCV49_RS27460
• Protein ID: WP_004213072.1
• Coordinates: 191185..191628 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: BCV49_RS27455
• Protein ID: WP_004213073.1
• Coordinates: 190958..191188 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCV49_RS27430	187181..188080	-	900	WP_004225022.1	nucleotide-binding protein	-
BCV49_RS27435	188070..188360	-	291	WP_004213078.1	hypothetical protein	-
BCV49_RS27440	188712..188918	+	207	WP_004213077.1	hypothetical protein	-
BCV49_RS27445	188908..189201	-	294	WP_004213076.1	hypothetical protein	-
BCV49_RS27450	189217..190350	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
BCV49_RS27455	190958..191188	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BCV49_RS27460	191185..191628	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCV49_RS27465	191642..192058	-	417	Protein_201	restriction endonuclease	-
BCV49_RS27470	192062..192355	-	294	Protein_202	transposase	-
BCV49_RS30445	192772..193408	+	637	Protein_203	response regulator	-
BCV49_RS29005	193923..194329	-	407	Protein_204	GAF domain-containing protein	-
BCV49_RS27480	194420..195340	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
BCV49_RS27485	195389..195880	-	492	WP_011251327.1	DM13 domain-containing protein	-
BCV49_RS27490	195943..196218	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / rmpA / iroN / iroD / iroC / iroB / rmpA2 / iutA / iucD / iucC / iucB / iucA	1..212770	212770

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T65151 WP_004213072.1 NZ_CP016815:191185-191628 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T65151 NZ_CP016815:191185-191628 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT65151 WP_004213073.1 NZ_CP016815:190958-191188 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT65151 NZ_CP016815:190958-191188 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620