Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 151754..152397 Replicon plasmid p_IncFIB_DHQP1002001
Accession NZ_CP016810
Organism Klebsiella pneumoniae strain DHQP1002001

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag A8C02_RS00785 Protein ID WP_000754566.1
Coordinates 151981..152397 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag A8C02_RS00780 Protein ID WP_001261276.1
Coordinates 151754..151984 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8C02_RS28090 146940..147413 + 474 WP_004152341.1 YkgJ family cysteine cluster protein -
A8C02_RS28095 147505..147735 + 231 WP_011977773.1 hypothetical protein -
A8C02_RS00750 148627..149409 - 783 WP_004152340.1 site-specific integrase -
A8C02_RS00755 149409..149741 - 333 WP_004152339.1 hypothetical protein -
A8C02_RS00760 149748..150146 - 399 WP_004171440.1 hypothetical protein -
A8C02_RS00765 150172..150501 - 330 WP_004152337.1 hypothetical protein -
A8C02_RS00770 150529..150837 - 309 WP_004152336.1 hypothetical protein -
A8C02_RS00780 151754..151984 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
A8C02_RS00785 151981..152397 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
A8C02_RS00790 152438..153315 - 878 Protein_158 restriction endonuclease -
A8C02_RS00795 153974..155251 + 1278 WP_016338369.1 HlyD family secretion protein -
A8C02_RS00800 155241..157349 + 2109 WP_016338368.1 peptidase domain-containing ABC transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(3'')-Ia / qacE / sul1 / aph(6)-Id / aph(3'')-Ib / sul2 / aac(6')-Ib-cr / dfrA14 - 1..307743 307743
- flank IS/Tn - - 145552..146820 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T65098 WP_000754566.1 NZ_CP016810:151981-152397 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T65098 NZ_CP016810:151981-152397 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT65098 WP_001261276.1 NZ_CP016810:151754-151984 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT65098 NZ_CP016810:151754-151984 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References