Detailed information of TA system TA065096

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 4425577..4426203
• Replicon: chromosome
• Accession: NZ_CP016809
• Organism: Paenibacillus ihbetae strain IHBB 9852

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W4ARZ2
• Locus tag: BBD41_RS19805
• Protein ID: WP_006212388.1
• Coordinates: 4425853..4426203 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: BBD41_RS19800
• Protein ID: WP_006212389.1
• Coordinates: 4425577..4425849 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBD41_RS19780	4420645..4421151	+	507	WP_007132377.1	winged helix-turn-helix domain-containing protein	-
BBD41_RS19785	4421206..4422540	+	1335	WP_099478520.1	MFS transporter	-
BBD41_RS19790	4422764..4423876	+	1113	WP_077567969.1	outer membrane lipoprotein-sorting protein	-
BBD41_RS19795	4424146..4425333	+	1188	WP_099478521.1	alanine racemase	-
BBD41_RS19800	4425577..4425849	+	273	WP_006212389.1	ribbon-helix-helix protein, CopG family	Antitoxin
BBD41_RS19805	4425853..4426203	+	351	WP_006212388.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BBD41_RS19810	4426942..4429134	+	2193	WP_099480703.1	RNA-binding transcriptional accessory protein	-
BBD41_RS19815	4429614..4430189	-	576	WP_077567967.1	CGNR zinc finger domain-containing protein	-
BBD41_RS19820	4430354..4430824	+	471	WP_077567966.1	DinB family protein	-
BBD41_RS19825	4430936..4431064	-	129	WP_007132384.1	cortex morphogenetic protein CmpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12751.64 Da
• Isoelectric Point: 4.8265

>T65096 WP_006212388.1 NZ_CP016809:4425853-4426203 [Paenibacillus ihbetae]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAASHGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMSKVDDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T65096 NZ_CP016809:4425853-4426203 [Paenibacillus ihbetae]
GTGATCGTTAAACGGGGCGACGTATTTTTTGCGGATTTATCACCCGTTGTCGGTTCGGAGCAGGGAGGCGTAAGGCCGGT
CCTTGTCATTCAGAACGATATCGGCAATCGGTTTAGTCCGACGGTAATTGTTGCAGCCATTACGGCACAAATTCAAAAAG
CCAAGCTGCCGACCCATGTCGAAATCGACGCGGCGTCGCATGGATTTGACCGGGATTCCGTTATCTTGCTAGAGCAGATT
CGTACGATTGACAAGCAACGGCTAACCGATAAAATCACCCATCTTGACGACGAAATGATGAGCAAGGTGGATGATTCCCT
GCAGATCAGTCTGGGCTTGATCGATTTCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10500.04 Da
• Isoelectric Point: 6.4708

>AT65096 WP_006212389.1 NZ_CP016809:4425577-4425849 [Paenibacillus ihbetae]
MQNTKRIMISLPDHLLQEVDGIAQLENSNRSELIRQAMKLYLNERKKRYIRESMQRGYMEMAKINLTMASEAFHAEEDAD
NTLDRLVSGV

Nucleotide

• Length: 273 bp

>AT65096 NZ_CP016809:4425577-4425849 [Paenibacillus ihbetae]
ATGCAGAACACCAAACGAATCATGATCAGTTTACCGGATCATCTTTTGCAGGAAGTGGACGGGATCGCGCAGCTGGAGAA
TTCCAACCGCAGTGAGCTTATTAGGCAAGCCATGAAGTTGTACCTGAATGAACGGAAGAAGCGATATATTCGCGAATCCA
TGCAACGGGGTTATATGGAGATGGCCAAAATTAACCTAACCATGGCTTCCGAGGCGTTTCACGCGGAGGAAGATGCAGAC
AACACTCTAGACCGCTTAGTAAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A1S2EZN8