Detailed information of TA system TA065082

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3066505..3067192
• Replicon: chromosome
• Accession: NZ_CP016794
• Organism: Mycobacterium tuberculosis strain SCAID 320.0

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: BEE65_RS14565
• Protein ID: WP_003414064.1
• Coordinates: 3066797..3067192 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: BEE65_RS14560
• Protein ID: WP_003414061.1
• Coordinates: 3066505..3066771 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEE65_RS14535	3062144..3063046	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
BEE65_RS14540	3063115..3063867	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
BEE65_RS14545	3064111..3064386	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
BEE65_RS14550	3064383..3066005	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
BEE65_RS14555	3066092..3066508	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
BEE65_RS14560	3066505..3066771	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BEE65_RS14565	3066797..3067192	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
BEE65_RS14570	3067189..3067458	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
BEE65_RS14575	3067468..3068562	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
BEE65_RS14580	3068559..3068978	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
BEE65_RS14585	3069052..3069531	-	480	WP_003414073.1	dihydrofolate reductase	-
BEE65_RS14590	3069602..3070402	-	801	WP_003911953.1	thymidylate synthase	-
BEE65_RS14595	3070558..3071295	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T65082 WP_003414064.1 NZ_CP016794:c3067192-3066797 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T65082 NZ_CP016794:c3067192-3066797 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT65082 WP_003414061.1 NZ_CP016794:c3066771-3066505 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT65082 NZ_CP016794:c3066771-3066505 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64