Detailed information of TA system TA065078

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2865850..2866487
• Replicon: chromosome
• Accession: NZ_CP016794
• Organism: Mycobacterium tuberculosis strain SCAID 320.0

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0DMV7
• Locus tag: BEE65_RS13480
• Protein ID: WP_003413180.1
• Coordinates: 2865850..2866245 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ44
• Locus tag: BEE65_RS13485
• Protein ID: WP_003413183.1
• Coordinates: 2866242..2866487 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEE65_RS13445	2861254..2862465	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
BEE65_RS13450	2862592..2863251	+	660	WP_003902296.1	hypothetical protein	-
BEE65_RS13455	2863248..2863910	+	663	WP_003905878.1	hypothetical protein	-
BEE65_RS22185	2863907..2864184	+	278	Protein_2629	type II toxin-antitoxin system VapB family antitoxin	-
BEE65_RS13460	2864277..2864690	+	414	WP_003413164.1	PIN domain nuclease	-
BEE65_RS13465	2864729..2864986	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	-
BEE65_RS13470	2864983..2865360	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
BEE65_RS13475	2865376..2865750	-	375	WP_003413177.1	hypothetical protein	-
BEE65_RS13480	2865850..2866245	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	Toxin
BEE65_RS13485	2866242..2866487	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
BEE65_RS13490	2866898..2867317	-	420	WP_003413190.1	A24 family peptidase	-
BEE65_RS13495	2867329..2868138	-	810	WP_003413193.1	shikimate dehydrogenase	-
BEE65_RS13500	2868135..2869388	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
BEE65_RS13505	2869381..2869893	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endonuclease VapC20-like (1-129)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-49)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.53 Da
• Isoelectric Point: 6.9794

>T65078 WP_003413180.1 NZ_CP016794:c2866245-2865850 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Nucleotide

• Length: 396 bp

>T65078 NZ_CP016794:c2866245-2865850 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9098.33 Da
• Isoelectric Point: 5.6007

>AT65078 WP_003413183.1 NZ_CP016794:c2866487-2866242 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Nucleotide

• Length: 246 bp

>AT65078 NZ_CP016794:c2866487-2866242 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• PDB: 5WZ4
• PDB: 5WZF

Antitoxin

• AlphaFold DB: A0A7U4BW31