Detailed information of TA system TA065054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1237396..1237964
• Replicon: chromosome
• Accession: NZ_CP016794
• Organism: Mycobacterium tuberculosis strain SCAID 320.0

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: BEE65_RS05885
• Protein ID: WP_003405865.1
• Coordinates: 1237590..1237964 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: BEE65_RS05880
• Protein ID: WP_003405863.1
• Coordinates: 1237396..1237593 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEE65_RS05860	1233437..1234075	-	639	WP_003898730.1	lipid droplet-associated protein	-
BEE65_RS05865	1234165..1235172	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
BEE65_RS05870	1235189..1236172	-	984	WP_003898731.1	hypothetical protein	-
BEE65_RS05875	1236235..1237308	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
BEE65_RS05880	1237396..1237593	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BEE65_RS05885	1237590..1237964	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
BEE65_RS05890	1238167..1238865	+	699	WP_003898733.1	hypothetical protein	-
BEE65_RS05895	1238983..1239168	+	186	WP_003901093.1	hypothetical protein	-
BEE65_RS05900	1239095..1239370	-	276	WP_003405867.1	hypothetical protein	-
BEE65_RS05905	1239613..1239936	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
BEE65_RS05910	1239951..1240661	-	711	WP_003911399.1	hypothetical protein	-
BEE65_RS22435	1240683..1240892	+	210	WP_003911400.1	hypothetical protein	-
BEE65_RS05920	1240844..1241551	-	708	Protein_1159	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T65054 WP_003405865.1 NZ_CP016794:1237590-1237964 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T65054 NZ_CP016794:1237590-1237964 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT65054 WP_003405863.1 NZ_CP016794:1237396-1237593 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT65054 NZ_CP016794:1237396-1237593 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07