Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2594084..2594720 Replicon chromosome
Accession NZ_CP016784
Organism Bacillus pumilus strain PDSLzg-1

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag BEN31_RS13345 Protein ID WP_003214169.1
Coordinates 2594084..2594434 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A267X601
Locus tag BEN31_RS13350 Protein ID WP_012008995.1
Coordinates 2594439..2594720 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEN31_RS13305 2589114..2589713 - 600 WP_034663944.1 SpoIIE family protein phosphatase -
BEN31_RS13310 2589713..2590501 - 789 WP_012009000.1 RNA polymerase sigma factor SigB -
BEN31_RS13315 2590467..2590955 - 489 WP_060595595.1 anti-sigma B factor RsbW -
BEN31_RS13320 2590952..2591281 - 330 WP_060595594.1 anti-sigma factor antagonist -
BEN31_RS13325 2591341..2592348 - 1008 WP_060595593.1 PP2C family protein-serine/threonine phosphatase -
BEN31_RS13330 2592359..2592760 - 402 WP_060595592.1 anti-sigma regulatory factor -
BEN31_RS13335 2592763..2593131 - 369 WP_003214235.1 RsbT antagonist protein RsbS -
BEN31_RS13340 2593091..2593966 - 876 WP_045210228.1 RsbT co-antagonist protein RsbRA -
BEN31_RS13345 2594084..2594434 - 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BEN31_RS13350 2594439..2594720 - 282 WP_012008995.1 hypothetical protein Antitoxin
BEN31_RS13355 2595014..2596195 - 1182 WP_066031291.1 alanine racemase -
BEN31_RS13360 2596336..2597352 - 1017 WP_106037124.1 outer membrane lipoprotein carrier protein LolA -
BEN31_RS13365 2597513..2597878 - 366 WP_034663960.1 holo-ACP synthase -
BEN31_RS13370 2597973..2598578 + 606 WP_060595589.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T65031 WP_003214169.1 NZ_CP016784:c2594434-2594084 [Bacillus pumilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T65031 NZ_CP016784:c2594434-2594084 [Bacillus pumilus]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCAGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATCAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACAATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10894.27 Da        Isoelectric Point: 5.2726

>AT65031 WP_012008995.1 NZ_CP016784:c2594720-2594439 [Bacillus pumilus]
MSESSATNEMKIRLPETLMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT65031 NZ_CP016784:c2594720-2594439 [Bacillus pumilus]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGACTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAGTTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A267X601

References