Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 201499..202134 Replicon chromosome
Accession NZ_CP016761
Organism Fictibacillus arsenicus strain G25-54

Toxin (Protein)


Gene name pemK Uniprot ID A0A165NT67
Locus tag ABE41_RS01175 Protein ID WP_066239453.1
Coordinates 201784..202134 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A4Q7Q5B3
Locus tag ABE41_RS01170 Protein ID WP_066285713.1
Coordinates 201499..201780 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ABE41_RS01145 196841..197587 + 747 WP_066285701.1 hypothetical protein -
ABE41_RS01150 197633..198238 - 606 WP_066285702.1 rhomboid family intramembrane serine protease -
ABE41_RS01155 198350..198709 + 360 WP_066285705.1 holo-ACP synthase -
ABE41_RS01160 198835..199848 + 1014 WP_066285709.1 outer membrane lipoprotein carrier protein LolA -
ABE41_RS01165 200182..201357 + 1176 WP_156774196.1 alanine racemase -
ABE41_RS01170 201499..201780 + 282 WP_066285713.1 antitoxin Antitoxin
ABE41_RS01175 201784..202134 + 351 WP_066239453.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ABE41_RS01180 202351..203154 + 804 WP_156774327.1 STAS domain-containing protein -
ABE41_RS01185 203161..203526 + 366 WP_171978977.1 STAS domain-containing protein -
ABE41_RS01190 203529..203930 + 402 WP_066285716.1 anti-sigma regulatory factor -
ABE41_RS01195 203918..204949 + 1032 WP_066285718.1 PP2C family protein-serine/threonine phosphatase -
ABE41_RS01200 205012..205344 + 333 WP_066285720.1 STAS domain-containing protein -
ABE41_RS01205 205341..205823 + 483 WP_066285725.1 anti-sigma B factor RsbW -
ABE41_RS01210 205789..206580 + 792 WP_066285727.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13028.04 Da        Isoelectric Point: 5.1617

>T65030 WP_066239453.1 NZ_CP016761:201784-202134 [Fictibacillus arsenicus]
MIVKRGDVYFADLSPVVGSEQGGIRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDSKKYGFERDSVILLEQI
RTIDKQRLTDKITQLDEDMMRRVDDALQISTGLVDF

Download         Length: 351 bp

>T65030 NZ_CP016761:201784-202134 [Fictibacillus arsenicus]
TTGATAGTCAAACGCGGAGACGTTTACTTTGCAGATCTTTCACCGGTAGTAGGTTCAGAACAAGGCGGAATACGTCCTGT
ACTGATAATCCAGAATGATATCGGAAACCGTTTTAGCCCAACAGTAATAGTAGCTGCAATCACAGCCCAAATTCAAAAAG
CAAAACTTCCAACACATGTTGAAATAGATTCTAAAAAATATGGCTTTGAACGGGATTCAGTTATTTTGCTTGAGCAGATC
CGAACAATTGACAAGCAAAGATTAACAGATAAGATTACACAGCTGGATGAAGACATGATGCGCAGGGTAGACGACGCATT
ACAGATCAGTACTGGTCTGGTAGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10981.68 Da        Isoelectric Point: 6.4796

>AT65030 WP_066285713.1 NZ_CP016761:201499-201780 [Fictibacillus arsenicus]
VSELNTKRIVISLPQKLLTEVDRVIKKENLDRSEFIHQATEMFLRERKNKRQFRDEMKQGYMEMAKINLTIASEAFMLEE
EADHTLDRLVSGV

Download         Length: 282 bp

>AT65030 NZ_CP016761:201499-201780 [Fictibacillus arsenicus]
GTGTCCGAATTGAACACAAAAAGAATCGTGATTAGTCTTCCTCAAAAGTTGCTGACTGAGGTAGATCGTGTCATTAAAAA
AGAGAATTTGGACCGCAGCGAATTTATCCATCAAGCTACTGAAATGTTTCTTCGTGAGCGGAAGAACAAACGTCAGTTTC
GTGATGAGATGAAACAGGGTTACATGGAAATGGCTAAGATCAATCTAACGATTGCCTCAGAAGCATTTATGTTAGAGGAG
GAAGCCGATCATACCTTGGACCGCTTAGTTAGCGGGGTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A165NT67

Antitoxin

Source ID Structure
AlphaFold DB A0A4Q7Q5B3

References