Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 655479..655993 Replicon chromosome
Accession NZ_CP016760
Organism Staphylococcus carnosus strain LTH 3730 SK 13 JCM 6069

Toxin (Protein)


Gene name mazF Uniprot ID A0A3G4R0E2
Locus tag BEK99_RS03170 Protein ID WP_075140914.1
Coordinates 655643..655993 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID B9DMI8
Locus tag BEK99_RS03165 Protein ID WP_015900820.1
Coordinates 655479..655646 (+) Length 56 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BEK99_RS13330 651559..651855 + 297 WP_162272169.1 PH domain-containing protein -
BEK99_RS03145 651848..653335 + 1488 WP_015900824.1 PH domain-containing protein -
BEK99_RS03150 653340..653825 + 486 WP_053464712.1 PH domain-containing protein -
BEK99_RS03155 653827..654180 + 354 WP_015900822.1 holo-ACP synthase -
BEK99_RS03160 654244..655398 + 1155 WP_065865267.1 alanine racemase -
BEK99_RS03165 655479..655646 + 168 WP_015900820.1 hypothetical protein Antitoxin
BEK99_RS03170 655643..655993 + 351 WP_075140914.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BEK99_RS03175 656337..657338 + 1002 WP_015900818.1 PP2C family protein-serine/threonine phosphatase -
BEK99_RS03180 657414..657740 + 327 WP_015900817.1 anti-sigma factor antagonist -
BEK99_RS03185 657742..658221 + 480 WP_015900816.1 anti-sigma B factor RsbW -
BEK99_RS03190 658196..658966 + 771 WP_015900815.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13075.18 Da        Isoelectric Point: 10.0708

>T65028 WP_075140914.1 NZ_CP016760:655643-655993 [Staphylococcus carnosus]
MMRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TVDKKRLKEKLTYLSDEKMKEIDNAIQISLGLTHRN

Download         Length: 351 bp

>T65028 NZ_CP016760:655643-655993 [Staphylococcus carnosus]
ATGATGAGGAGAGGCGATGTTTACTTAGCTGATTTATCACCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGGCCTGTTGT
AATTATACAAAATGACACTGGAAATAAGTATAGTCCGACAGTTATTGTGGCAGCAATTACTGGACGGATTAATAAAGCAA
AAATTCCTACTCATGTTGAAATTGAAAAAAGTAAATATAAATTGGACAAAGATTCTGTTATATTACTTGAACAGATTAGA
ACAGTCGATAAAAAGCGACTTAAAGAAAAACTGACATATTTATCAGACGAAAAGATGAAAGAAATTGATAATGCGATTCA
AATCAGCTTAGGCTTGACACATCGCAATTAA

Antitoxin


Download         Length: 56 a.a.        Molecular weight: 6211.75 Da        Isoelectric Point: 3.6836

>AT65028 WP_015900820.1 NZ_CP016760:655479-655646 [Staphylococcus carnosus]
MSTFNQNRSYSLEQSLKEGYAQMADLNLSLATEAFSVECEACDCNESYLAFDKDE

Download         Length: 168 bp

>AT65028 NZ_CP016760:655479-655646 [Staphylococcus carnosus]
ATGTCAACTTTTAATCAGAATAGAAGTTATAGTTTAGAACAGTCACTGAAAGAAGGCTATGCACAGATGGCTGACTTAAA
TCTCTCCCTAGCAACGGAGGCATTTTCAGTAGAATGTGAAGCCTGTGATTGCAATGAATCATATTTAGCTTTTGACAAGG
ACGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G4R0E2

Antitoxin

Source ID Structure
AlphaFold DB A0A380F5Y4

References