Detailed information of TA system TA065027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 642118..642629
• Replicon: chromosome
• Accession: NZ_CP016760
• Organism: Staphylococcus carnosus strain LTH 3730 SK 13 JCM 6069

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: B9DMH5
• Locus tag: BEK99_RS03105
• Protein ID: WP_015900832.1
• Coordinates: 642423..642629 (+)
• Length: 69 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: -
• Locus tag: BEK99_RS03100
• Protein ID: WP_065865261.1
• Coordinates: 642118..642411 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BEK99_RS03075	637488..638126	+	639	WP_015900838.1	thiamine phosphate synthase	-
BEK99_RS03080	638215..639084	+	870	WP_065865259.1	membrane protein insertase YidC	-
BEK99_RS03085	639195..639708	+	514	Protein_609	GNAT family N-acetyltransferase	-
BEK99_RS03090	639799..640446	-	648	WP_065865260.1	HD domain-containing protein	-
BEK99_RS03095	640471..641953	-	1483	Protein_611	cardiolipin synthase	-
BEK99_RS03100	642118..642411	+	294	WP_065865261.1	copper-sensing transcriptional repressor CsoR	Antitoxin
BEK99_RS03105	642423..642629	+	207	WP_015900832.1	cation transporter	Toxin
BEK99_RS03110	642644..642853	+	210	WP_015900831.1	copper ion binding protein	-
BEK99_RS03115	642950..645151	+	2202	WP_065865262.1	heavy metal translocating P-type ATPase	-
BEK99_RS13385	645227..645367	+	141	WP_167313208.1	hypothetical protein	-
BEK99_RS03120	645440..646657	-	1218	WP_015900829.1	rod shape-determining protein RodA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (27-65)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7833.72 Da
• Isoelectric Point: 4.1353

>T65027 WP_015900832.1 NZ_CP016760:642423-642629 [Staphylococcus carnosus]
MQNSVIKIDGMETEEEQHKLHQHLIEMTGVSAVQVDMDSNEVRISYETPVNLNNLEKEIYDAGYQILN

Nucleotide

• Length: 207 bp

>T65027 NZ_CP016760:642423-642629 [Staphylococcus carnosus]
ATGCAAAATAGTGTAATTAAAATTGATGGAATGGAAACTGAAGAAGAACAGCATAAATTGCATCAACATCTTATAGAAAT
GACAGGTGTATCTGCAGTGCAAGTGGATATGGATTCAAATGAAGTCAGAATCAGTTATGAAACACCTGTAAATCTCAATA
ATCTTGAAAAAGAAATCTATGATGCAGGTTATCAAATATTAAATTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11132.76 Da
• Isoelectric Point: 6.4862

>AT65027 WP_065865261.1 NZ_CP016760:642118-642411 [Staphylococcus carnosus]
MTENDKAHHSEQLKSNLKARLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAVRLLDHHMKGCIMHKVEDGHA
EEAMEELLVTVQKLIKD

Nucleotide

• Length: 294 bp

>AT65027 NZ_CP016760:642118-642411 [Staphylococcus carnosus]
ATGACCGAAAATGATAAAGCGCACCATTCAGAACAACTCAAATCCAACTTGAAAGCACGACTCAATCGAATTGAAGGACA
AGTCAGAGCAATTAACCGAATGATTGAAGAGGATGTATATTGTGATGATGTACTCACACAAATCCGTGCAACACGTTCAG
CTTTGAACAGTGTGGCAGTACGATTGCTTGATCATCACATGAAAGGTTGTATCATGCATAAAGTTGAGGATGGTCACGCT
GAAGAAGCGATGGAAGAATTGCTTGTTACCGTTCAAAAATTGATTAAAGATTAA

Structures

Toxin

• AlphaFold DB: A0A380F432