Detailed information of TA system TA065026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2305272..2305843
• Replicon: chromosome
• Accession: NZ_CP016757
• Organism: Cloacibacillus porcorum strain CL-84 (T)

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1B2I645
• Locus tag: BED41_RS10335
• Protein ID: WP_066745698.1
• Coordinates: 2305529..2305843 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1B2I624
• Locus tag: BED41_RS10330
• Protein ID: WP_066745693.1
• Coordinates: 2305272..2305535 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BED41_RS10310	2300530..2301075	+	546	WP_066745684.1	CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase	-
BED41_RS10315	2301408..2302496	-	1089	WP_066745687.1	tryptophan--tRNA ligase	-
BED41_RS10320	2302601..2303194	-	594	WP_066745689.1	glycerol-3-phosphate 1-O-acyltransferase PlsY	-
BED41_RS10325	2303492..2304856	+	1365	WP_066745691.1	META domain-containing protein	-
BED41_RS16185	2304993..2305211	-	219	WP_084002400.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BED41_RS10330	2305272..2305535	+	264	WP_066745693.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BED41_RS10335	2305529..2305843	+	315	WP_066745698.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BED41_RS10340	2306141..2307352	+	1212	WP_066745701.1	site-specific integrase	-
BED41_RS10345	2307372..2307779	-	408	WP_066745704.1	type II toxin-antitoxin system HicB family antitoxin	-
BED41_RS10350	2307824..2308030	-	207	WP_008710032.1	type II toxin-antitoxin system HicA family toxin	-
BED41_RS10355	2308199..2309008	-	810	WP_066745707.1	site-specific DNA-methyltransferase	-
BED41_RS10360	2309067..2309480	-	414	WP_157102324.1	hypothetical protein	-
BED41_RS10365	2309362..2309745	-	384	WP_066745713.1	hypothetical protein	-
BED41_RS10370	2309745..2310014	-	270	WP_066745716.1	hypothetical protein	-
BED41_RS10375	2310014..2310241	-	228	WP_066745718.1	hypothetical protein	-
BED41_RS10380	2310243..2310830	-	588	WP_066745720.1	N-acetylmuramoyl-L-alanine amidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-97)

Antitoxin

• Antitoxin PemI-like (1-87)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11694.62 Da
• Isoelectric Point: 6.6341

>T65026 WP_066745698.1 NZ_CP016757:2305529-2305843 [Cloacibacillus porcorum]
MVSQGDIIWLEFNPQAGHRPAVVVSNNIFNNVTGMAIVCPITSKLKAFPLHIPLDERTKTQGMIACQHIKSLDIAARNYR
HIEKLPHDILDRVLEAVLSEIEEI

Nucleotide

• Length: 315 bp

>T65026 NZ_CP016757:2305529-2305843 [Cloacibacillus porcorum]
ATGGTAAGCCAGGGGGATATCATCTGGCTGGAGTTTAACCCGCAGGCAGGACACCGCCCCGCCGTCGTGGTGAGCAATAA
CATCTTCAACAATGTTACTGGAATGGCCATCGTCTGCCCGATTACAAGTAAGCTGAAGGCCTTCCCTTTGCATATACCGC
TTGACGAACGGACGAAAACACAGGGGATGATCGCCTGCCAGCATATTAAATCTTTGGATATTGCGGCGAGGAATTACCGG
CATATCGAGAAGCTGCCGCATGATATTCTGGATCGTGTGCTGGAGGCGGTCCTATCTGAGATCGAAGAGATTTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9739.32 Da
• Isoelectric Point: 8.5162

>AT65026 WP_066745693.1 NZ_CP016757:2305272-2305535 [Cloacibacillus porcorum]
MQTMIQKWGNSQGIRLPKHILESVGLEIPATGKPVEVEVIAEEGKITIKKPLPSRKRRNIIELLAGYEGDYKASETDWGV
PVGKEVW

Nucleotide

• Length: 264 bp

>AT65026 NZ_CP016757:2305272-2305535 [Cloacibacillus porcorum]
ATGCAGACAATGATCCAAAAGTGGGGCAATAGCCAGGGGATCAGGCTGCCAAAGCATATTTTAGAATCAGTAGGTCTTGA
AATACCGGCGACAGGCAAGCCTGTGGAGGTGGAGGTTATCGCCGAAGAGGGTAAGATAACGATCAAGAAGCCGCTTCCCA
GCCGCAAGCGCAGGAACATTATTGAACTTTTGGCCGGTTATGAGGGCGATTATAAGGCTTCGGAGACAGATTGGGGAGTC
CCCGTAGGTAAGGAAGTATGGTAA

Structures

Toxin

• AlphaFold DB: A0A1B2I645

Antitoxin

• AlphaFold DB: A0A1B2I624