Detailed information of TA system TA064984

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii(toxin)
• Location: 39523..41279
• Replicon: plasmid p275A
• Accession: NZ_CP016699
• Organism: Lactococcus lactis subsp. lactis strain 275

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: Q48616
• Locus tag: LL275_RS12920
• Protein ID: WP_012477748.1
• Coordinates: 39523..40419 (-)
• Length: 299 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: Q48615
• Locus tag: LL275_RS12925
• Protein ID: WP_012477749.1
• Coordinates: 40416..41279 (-)
• Length: 288 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LL275_RS12895	37122..37634	-	513	Protein_44	transposase	-
LL275_RS12900	37636..37881	-	246	Protein_45	hypothetical protein	-
LL275_RS14270	37929..39076	+	1148	Protein_46	IS3 family transposase	-
LL275_RS12920	39523..40419	-	897	WP_012477748.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LL275_RS12925	40416..41279	-	864	WP_012477749.1	AbiEi	Antitoxin
LL275_RS12930	41632..42213	-	582	WP_081144401.1	recombinase family protein	-
LL275_RS12935	42485..43321	-	837	WP_023349284.1	AraC family transcriptional regulator	-
LL275_RS12940	43609..43944	+	336	WP_011915238.1	bacteriocin immunity protein	-
LL275_RS12945	44081..44395	+	315	Protein_52	Nif3-like dinuclear metal center hexameric protein	-
LL275_RS12950	44435..44800	-	366	WP_021166316.1	hypothetical protein	-
LL275_RS12955	44925..45176	-	252	Protein_54	HAD hydrolase family protein	-
LL275_RS14420	45104..45271	-	168	WP_161487267.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92710	92710

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (30-250)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 299 a.a.
• Molecular weight: 35372.47 Da
• Isoelectric Point: 5.2182

>T64984 WP_012477748.1 NZ_CP016699:c40419-39523 [Lactococcus lactis subsp. lactis]
MKNTRLKDLIATRNDDIGIENYRIRYATERFLTRLSASQYKEKFVLKGGFLIGVTYNLSQRTTKDLDTALIDFKSDAQSI
ERVITEICNIDLEDQVLFKLKELTSSQDMRIYPGYRAKLKMMFPDGNTRIDFDLDIGVGDRITPEAKKIKIPLIFNEVKG
VEKQIEVLAYPKETIQAEKLETILTRGKVNTRMKDYYDFHLLLTDQENSNSISFYYAFKNTWEFRNPTQFIDEELFEDWL
FILDEILESKELKEKYWPNYIKDRNYAKHLNMDDIISEIKEFVSKLKEEYIKENMSRN

Nucleotide

• Length: 897 bp

>T64984 NZ_CP016699:c40419-39523 [Lactococcus lactis subsp. lactis]
ATGAAAAATACAAGGTTAAAGGATTTAATTGCAACAAGAAATGATGATATTGGAATTGAAAATTATAGAATAAGATATGC
AACTGAAAGATTCTTAACAAGGCTTTCGGCAAGTCAATATAAGGAAAAATTTGTTTTAAAAGGAGGTTTTTTAATTGGTG
TCACCTATAACTTGAGTCAGAGGACTACTAAGGATTTAGATACAGCTCTGATAGACTTTAAAAGTGATGCACAGTCTATT
GAACGGGTAATTACTGAAATATGTAATATAGATTTAGAAGACCAAGTGCTTTTTAAATTAAAGGAACTTACAAGCAGTCA
AGATATGAGAATATATCCTGGGTACAGAGCTAAACTTAAGATGATGTTTCCTGATGGAAATACAAGGATAGACTTTGATC
TTGATATTGGAGTAGGAGATAGGATAACCCCAGAGGCTAAGAAAATAAAAATACCTCTAATATTTAATGAAGTAAAAGGT
GTAGAGAAACAGATAGAAGTATTAGCTTATCCAAAAGAAACTATTCAGGCAGAAAAATTAGAGACAATTCTCACTCGGGG
GAAAGTAAATACTAGAATGAAAGATTATTACGATTTTCATTTGCTTTTGACTGATCAAGAAAATAGTAATAGTATAAGTT
TTTATTATGCTTTTAAAAATACTTGGGAATTTAGAAACCCAACTCAATTTATTGATGAAGAACTGTTTGAAGATTGGCTC
TTTATTTTAGATGAGATACTTGAATCAAAAGAATTGAAAGAAAAGTATTGGCCAAACTATATTAAAGATAGAAATTATGC
TAAACATCTAAACATGGATGATATCATTTCAGAAATTAAAGAATTTGTTAGTAAATTAAAAGAAGAATATATAAAGGAGA
ATATGTCTAGAAATTAA

Antitoxin

Protein

• Length: 288 a.a.
• Molecular weight: 33815.92 Da
• Isoelectric Point: 8.1894

>AT64984 WP_012477749.1 NZ_CP016699:c41279-40416 [Lactococcus lactis subsp. lactis]
MDTYRISNLNLEQEDINNLKRISTNMLDTFNHEQLLSIIDVMKNTYFMNELSTYLVDDNLPDVGTEEFNFLVLANKYKGN
IIRKIVRDEGISDYYLRKFVLKYNLTEVDKGVYIFPHKKKDSLFIFQQKYSKAVISHETSLYLQDVIDYIPQKIQMSVPE
KYNISRIQEPHENRLTSYNYVDINSNNIMDKNIPINLVRNKSISPTQIETVNSFLGLPLRVTSIARSIVDVLKPSHKAEE
EVKEQAIKYYLERFPDNIVRLKRIAKTQNVLKELEYYLILLGVHYKL

Nucleotide

• Length: 864 bp

>AT64984 NZ_CP016699:c41279-40416 [Lactococcus lactis subsp. lactis]
ATGGACACTTATAGAATATCAAACTTAAATCTAGAACAGGAAGATATAAATAATCTAAAGAGAATCTCAACCAATATGTT
AGATACTTTTAACCATGAACAGCTTCTTTCAATCATTGATGTGATGAAAAATACTTATTTCATGAATGAACTCTCAACCT
ATTTAGTAGACGATAATCTCCCTGATGTAGGTACTGAAGAATTTAATTTTTTGGTTCTTGCTAATAAATATAAAGGAAAT
ATAATAAGAAAAATAGTACGTGATGAAGGTATAAGTGATTACTATTTGAGAAAATTTGTTTTGAAGTATAATCTTACTGA
GGTTGACAAAGGTGTTTATATTTTTCCTCATAAAAAAAAGGACAGTTTATTTATTTTTCAGCAGAAATACAGCAAAGCAG
TTATCTCACATGAAACATCATTATATCTACAAGATGTAATAGATTATATTCCGCAAAAAATACAAATGAGCGTTCCAGAA
AAGTATAATATCAGTAGAATTCAGGAACCTCACGAAAATCGTTTAACAAGCTATAACTATGTGGATATTAACTCTAATAA
TATAATGGATAAAAATATTCCAATCAATTTAGTCAGAAATAAGAGTATTAGTCCTACACAAATAGAAACAGTAAATAGCT
TTTTAGGTCTCCCACTAAGAGTCACTTCTATTGCTCGGTCGATAGTAGACGTTTTAAAACCTTCCCACAAGGCTGAAGAA
GAAGTGAAAGAACAGGCGATTAAGTATTATTTAGAAAGATTTCCAGATAATATTGTGCGCTTAAAACGTATAGCTAAAAC
ACAAAATGTTTTAAAAGAACTAGAGTATTACTTGATTTTATTGGGAGTACATTATAAACTATGA

Structures

Toxin

• AlphaFold DB: Q48616

Antitoxin

• AlphaFold DB: Q48615