Detailed information of TA system TA064948

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 196810..197251
• Replicon: chromosome
• Accession: NZ_CP016639
• Organism: Burkholderia pseudomallei strain M1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: A7U58_RS18790
• Protein ID: WP_017881988.1
• Coordinates: 197078..197251 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: A7U58_RS18785
• Protein ID: WP_004525420.1
• Coordinates: 196810..197094 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7U58_RS18765	191918..193180	-	1263	WP_017881884.1	c-type cytochrome	-
A7U58_RS18770	193339..194799	-	1461	WP_004535188.1	metallophosphoesterase	-
A7U58_RS18775	195046..195162	+	117	Protein_146	IS3 family transposase	-
A7U58_RS35130	195389..195817	-	429	Protein_147	IS110 family transposase	-
A7U58_RS18780	195780..196163	-	384	Protein_148	DDE-type integrase/transposase/recombinase	-
A7U58_RS18785	196810..197094	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
A7U58_RS18790	197078..197251	-	174	WP_017881988.1	BrnT family toxin	Toxin
A7U58_RS18795	197918..198193	+	276	WP_004537565.1	bacteriophage protein Gp49	-
A7U58_RS18800	198203..198334	+	132	WP_009923452.1	bacteriophage protein Gp48	-
A7U58_RS18810	198470..198691	+	222	WP_004552390.1	hypothetical protein	-
A7U58_RS40125	198658..198957	+	300	WP_160458000.1	hypothetical protein	-
A7U58_RS18820	199188..199769	-	582	WP_004542548.1	hypothetical protein	-
A7U58_RS18825	199820..200407	+	588	Protein_156	prohibitin family protein	-
A7U58_RS40130	200405..200767	+	363	Protein_157	DDE-type integrase/transposase/recombinase	-
A7U58_RS35145	200829..201964	-	1136	Protein_158	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	189696..211790	22094
	-	inside	IScluster/Tn	-	-	195780..204347	8567

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6663.54 Da
• Isoelectric Point: 7.9950

>T64948 WP_017881988.1 NZ_CP016639:c197251-197078 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREAKSYVEQA

Nucleotide

• Length: 174 bp

>T64948 NZ_CP016639:c197251-197078 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGCCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT64948 WP_004525420.1 NZ_CP016639:c197094-196810 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT64948 NZ_CP016639:c197094-196810 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8