Detailed information of TA system TA064947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 3960522..3961177
• Replicon: chromosome
• Accession: NZ_CP016638
• Organism: Burkholderia pseudomallei strain M1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0F6LEW6
• Locus tag: A7U58_RS17565
• Protein ID: WP_006027262.1
• Coordinates: 3960881..3961177 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q63YL3
• Locus tag: A7U58_RS17560
• Protein ID: WP_004202809.1
• Coordinates: 3960522..3960878 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7U58_RS34855	3955863..3956186	+	324	WP_094188790.1	helix-turn-helix domain-containing protein	-
A7U58_RS34860	3956273..3956842	+	570	WP_082250078.1	DUF4433 domain-containing protein	-
A7U58_RS17540	3956842..3957486	+	645	WP_059642481.1	DUF4433 domain-containing protein	-
A7U58_RS17545	3957486..3958562	+	1077	WP_065793620.1	macro domain-containing protein	-
A7U58_RS17550	3958929..3959426	+	498	WP_004199888.1	terminase	-
A7U58_RS17555	3959423..3960478	+	1056	WP_041192239.1	phage portal protein	-
A7U58_RS17560	3960522..3960878	-	357	WP_004202809.1	putative addiction module antidote protein	Antitoxin
A7U58_RS17565	3960881..3961177	-	297	WP_006027262.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A7U58_RS34865	3961845..3963101	+	1257	WP_004552667.1	AAA family ATPase	-
A7U58_RS17575	3963104..3963721	+	618	WP_031313497.1	DUF4276 family protein	-
A7U58_RS17580	3964493..3964819	+	327	WP_004552669.1	hypothetical protein	-
A7U58_RS17585	3964812..3965225	+	414	WP_065793621.1	phage tail protein I	-
A7U58_RS17590	3965123..3965725	+	603	WP_065793622.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3942954..3968466	25512

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10844.65 Da
• Isoelectric Point: 10.1723

>T64947 WP_006027262.1 NZ_CP016638:c3961177-3960881 [Burkholderia pseudomallei]
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRRGKIIVVVLCGGDKS
TQKKDIKLAKQIAGELED

Nucleotide

• Length: 297 bp

>T64947 NZ_CP016638:c3961177-3960881 [Burkholderia pseudomallei]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCAATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTATTTCGTGCGGCGCGGAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGGCGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12585.41 Da
• Isoelectric Point: 9.9470

>AT64947 WP_004202809.1 NZ_CP016638:c3960878-3960522 [Burkholderia pseudomallei]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT64947 NZ_CP016638:c3960878-3960522 [Burkholderia pseudomallei]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGAAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A0F6LEW6

Antitoxin

• AlphaFold DB: A0A3N4G1D3