Detailed information of TA system TA064946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3956842..3958562
• Replicon: chromosome
• Accession: NZ_CP016638
• Organism: Burkholderia pseudomallei strain M1

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: A7U58_RS17540
• Protein ID: WP_059642481.1
• Coordinates: 3956842..3957486 (+)
• Length: 215 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: A7U58_RS17545
• Protein ID: WP_065793620.1
• Coordinates: 3957486..3958562 (+)
• Length: 359 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7U58_RS17535	3952267..3952497	+	231	WP_065793619.1	type I toxin-antitoxin system ptaRNA1 family toxin	-
A7U58_RS39280	3952528..3952683	-	156	WP_155122752.1	hypothetical protein	-
A7U58_RS39285	3953239..3953853	+	615	WP_129112001.1	hypothetical protein	-
A7U58_RS34845	3953853..3954977	+	1125	WP_082250077.1	ImmA/IrrE family metallo-endopeptidase	-
A7U58_RS34850	3955007..3955477	+	471	WP_080306021.1	hypothetical protein	-
A7U58_RS34855	3955863..3956186	+	324	WP_094188790.1	helix-turn-helix domain-containing protein	-
A7U58_RS34860	3956273..3956842	+	570	WP_082250078.1	DUF4433 domain-containing protein	-
A7U58_RS17540	3956842..3957486	+	645	WP_059642481.1	DUF4433 domain-containing protein	Toxin
A7U58_RS17545	3957486..3958562	+	1077	WP_065793620.1	macro domain-containing protein	Antitoxin
A7U58_RS17550	3958929..3959426	+	498	WP_004199888.1	terminase	-
A7U58_RS17555	3959423..3960478	+	1056	WP_041192239.1	phage portal protein	-
A7U58_RS17560	3960522..3960878	-	357	WP_004202809.1	putative addiction module antidote protein	-
A7U58_RS17565	3960881..3961177	-	297	WP_006027262.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A7U58_RS34865	3961845..3963101	+	1257	WP_004552667.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3942954..3968466	25512

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (14-214)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 215 a.a.
• Molecular weight: 24887.60 Da
• Isoelectric Point: 7.5391

>T64946 WP_059642481.1 NZ_CP016638:3956842-3957486 [Burkholderia pseudomallei]
MLYTSLNPEKALIWRIVHRDNLPWILDNGLYCGNSAVKAPAWVNIGNPELIDKRAHHPVPLPPRGLLNDYVPFYFTPFSP
MLRNIHTGWGGIQKRPNEEIVILVSSLHHIAGLGLPFLFTDSHAYYRWADYYSDLAYLDKIDWPLLQRRDFKRDPDDPAK
FERYQAEALIHQHLPVGGLLGIVCYTEPLKQRIEQEIQARNLNLPVYARTGWYF

Nucleotide

• Length: 645 bp

>T64946 NZ_CP016638:3956842-3957486 [Burkholderia pseudomallei]
ATGCTCTATACCAGCCTTAATCCGGAAAAAGCACTGATCTGGCGGATTGTCCACCGCGACAATCTGCCGTGGATTCTGGA
CAATGGCCTGTATTGCGGCAACAGCGCGGTGAAGGCGCCGGCATGGGTGAATATCGGTAACCCGGAGCTTATCGACAAGC
GCGCCCATCACCCGGTGCCGTTGCCGCCGCGCGGCTTGCTGAATGACTATGTACCGTTCTATTTCACGCCCTTCTCGCCG
ATGCTGCGTAACATTCACACCGGATGGGGCGGAATCCAGAAGCGGCCGAATGAAGAAATCGTGATCTTGGTGTCTAGCCT
GCACCACATTGCAGGGTTGGGCTTGCCGTTTTTGTTTACTGACAGTCATGCCTATTATCGTTGGGCAGATTACTACTCGG
ACTTGGCCTACCTCGATAAGATCGACTGGCCTTTGCTACAGCGAAGGGATTTCAAGCGTGACCCGGACGACCCCGCCAAG
TTCGAGCGCTATCAGGCGGAGGCGCTGATTCACCAACACTTGCCAGTGGGCGGACTTCTCGGCATCGTGTGCTATACGGA
ACCTTTGAAACAACGCATCGAACAGGAGATTCAGGCCCGTAATCTGAACCTGCCGGTTTATGCCCGAACAGGGTGGTACT
TCTGA

Antitoxin

Protein

• Length: 359 a.a.
• Molecular weight: 40560.52 Da
• Isoelectric Point: 6.0092

>AT64946 WP_065793620.1 NZ_CP016638:3957486-3958562 [Burkholderia pseudomallei]
MITFTQGNLLEARTEALVNTVNTVGVMGKGIALMFKERFDENFRRYAAACKAKEVQTGKMFVTPVHELDGPRWIVNFPTK
QHWRAPSRMEWVVEGLQDLRRFLIEQQVQSIAIPPLGAGNGGLEWADVREQIELALGDLDIDIVVFEPTKQYQNVAKRAG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERAIERFNPGENPLNLQFAPHKYGPYANRLDHLLNNLDGSYLH
SEKRIGDANPLDVIWFDDERKAFVQTYLKSEAKAYTQALEFTAALIDGFESPFGMELLATVDWLLTREGVAPNVPALREG
LRHWRGGSDAATRKDRLFDDRALGIALERLTQPATALV

Nucleotide

• Length: 1077 bp

>AT64946 NZ_CP016638:3957486-3958562 [Burkholderia pseudomallei]
ATGATTACATTCACTCAAGGCAATTTGTTGGAGGCCCGCACTGAGGCGCTGGTCAACACCGTCAACACCGTCGGGGTGAT
GGGCAAGGGCATCGCGCTGATGTTCAAGGAACGCTTTGATGAGAACTTCCGCCGCTATGCCGCCGCCTGCAAAGCGAAGG
AAGTGCAGACAGGCAAGATGTTTGTGACGCCGGTGCACGAACTCGATGGGCCGCGCTGGATCGTTAATTTCCCTACCAAG
CAGCACTGGCGTGCGCCCTCGCGCATGGAGTGGGTGGTGGAAGGCTTGCAGGACCTGCGGCGCTTCTTGATCGAGCAACA
GGTTCAATCCATCGCTATCCCGCCGCTGGGTGCGGGTAACGGTGGCCTGGAATGGGCTGATGTGCGTGAGCAGATCGAAC
TGGCGTTGGGCGATCTGGACATCGACATTGTGGTGTTCGAGCCCACCAAGCAATACCAGAACGTGGCCAAGCGTGCTGGC
GTAGAAAAGCTGACGCCCGCCCGAGCGCTGATCGCCGAATTGGTGCGCCGGTACTGGGTGCTGGGCATGGAATGCAGCTT
GCTGGAGATCCAGAAGCTGGCGTGGTTTCTGGAACGCGCCATTGAGCGTTTCAACCCAGGTGAAAACCCGCTGAACTTGC
AGTTTGCACCACACAAATACGGTCCCTACGCCAACCGACTTGACCACCTGTTGAACAATCTGGATGGCAGCTACCTGCAC
TCTGAAAAGCGCATCGGCGATGCCAATCCGCTGGACGTGATTTGGTTCGACGACGAACGCAAGGCTTTTGTTCAGACATA
TCTCAAGAGCGAAGCCAAGGCCTACACGCAAGCTCTTGAATTCACGGCGGCACTGATCGATGGCTTTGAATCACCCTTTG
GCATGGAACTGCTGGCGACCGTGGATTGGTTGCTGACCCGTGAAGGCGTGGCACCCAACGTGCCGGCCTTGCGCGAAGGG
CTCCGTCATTGGCGCGGTGGTTCAGATGCTGCCACCCGTAAGGACCGCCTTTTTGATGATCGGGCACTGGGCATCGCCCT
GGAGCGTCTGACACAGCCTGCGACAGCGCTGGTGTGA