Detailed information of TA system TA064943

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 3958063..3958718
• Replicon: chromosome
• Accession: NZ_CP016636
• Organism: Burkholderia pseudomallei strain MS

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0F6LEW6
• Locus tag: A7U59_RS17525
• Protein ID: WP_006027262.1
• Coordinates: 3958422..3958718 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q63YL3
• Locus tag: A7U59_RS17520
• Protein ID: WP_004202809.1
• Coordinates: 3958063..3958419 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7U59_RS34795	3953506..3953727	+	222	WP_080306026.1	helix-turn-helix domain-containing protein	-
A7U59_RS34800	3953814..3954383	+	570	WP_082250078.1	DUF4433 domain-containing protein	-
A7U59_RS17500	3954383..3955027	+	645	WP_059642481.1	DUF4433 domain-containing protein	-
A7U59_RS17505	3955027..3956103	+	1077	WP_065793620.1	macro domain-containing protein	-
A7U59_RS17510	3956470..3956967	+	498	WP_004199888.1	terminase	-
A7U59_RS17515	3956964..3958019	+	1056	WP_041192239.1	phage portal protein	-
A7U59_RS17520	3958063..3958419	-	357	WP_004202809.1	putative addiction module antidote protein	Antitoxin
A7U59_RS17525	3958422..3958718	-	297	WP_006027262.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A7U59_RS34805	3959386..3960642	+	1257	WP_004552667.1	AAA family ATPase	-
A7U59_RS17535	3960687..3961262	+	576	WP_198014201.1	DUF4276 family protein	-
A7U59_RS17540	3962034..3962360	+	327	WP_004552669.1	hypothetical protein	-
A7U59_RS17545	3962353..3962766	+	414	WP_065793621.1	phage tail protein I	-
A7U59_RS17550	3962664..3963266	+	603	WP_065793622.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3938248..3966007	27759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10844.65 Da
• Isoelectric Point: 10.1723

>T64943 WP_006027262.1 NZ_CP016636:c3958718-3958422 [Burkholderia pseudomallei]
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRRGKIIVVVLCGGDKS
TQKKDIKLAKQIAGELED

Nucleotide

• Length: 297 bp

>T64943 NZ_CP016636:c3958718-3958422 [Burkholderia pseudomallei]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCAATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTATTTCGTGCGGCGCGGAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGGCGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12585.41 Da
• Isoelectric Point: 9.9470

>AT64943 WP_004202809.1 NZ_CP016636:c3958419-3958063 [Burkholderia pseudomallei]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT64943 NZ_CP016636:c3958419-3958063 [Burkholderia pseudomallei]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGAAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A0F6LEW6

Antitoxin

• AlphaFold DB: A0A3N4G1D3