Detailed information of TA system TA064942

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3954383..3956103
• Replicon: chromosome
• Accession: NZ_CP016636
• Organism: Burkholderia pseudomallei strain MS

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: A7U59_RS17500
• Protein ID: WP_059642481.1
• Coordinates: 3954383..3955027 (+)
• Length: 215 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: A7U59_RS17505
• Protein ID: WP_065793620.1
• Coordinates: 3955027..3956103 (+)
• Length: 359 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7U59_RS17495	3949808..3950038	+	231	WP_065793619.1	type I toxin-antitoxin system ptaRNA1 family toxin	-
A7U59_RS40230	3950069..3950224	-	156	WP_155122752.1	hypothetical protein	-
A7U59_RS39295	3950780..3951394	+	615	WP_129112001.1	hypothetical protein	-
A7U59_RS34785	3951394..3952518	+	1125	WP_082250077.1	ImmA/IrrE family metallo-endopeptidase	-
A7U59_RS34790	3952548..3953018	+	471	WP_080306021.1	hypothetical protein	-
A7U59_RS34795	3953506..3953727	+	222	WP_080306026.1	helix-turn-helix domain-containing protein	-
A7U59_RS34800	3953814..3954383	+	570	WP_082250078.1	DUF4433 domain-containing protein	-
A7U59_RS17500	3954383..3955027	+	645	WP_059642481.1	DUF4433 domain-containing protein	Toxin
A7U59_RS17505	3955027..3956103	+	1077	WP_065793620.1	macro domain-containing protein	Antitoxin
A7U59_RS17510	3956470..3956967	+	498	WP_004199888.1	terminase	-
A7U59_RS17515	3956964..3958019	+	1056	WP_041192239.1	phage portal protein	-
A7U59_RS17520	3958063..3958419	-	357	WP_004202809.1	putative addiction module antidote protein	-
A7U59_RS17525	3958422..3958718	-	297	WP_006027262.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A7U59_RS34805	3959386..3960642	+	1257	WP_004552667.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3938248..3966007	27759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (14-214)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 215 a.a.
• Molecular weight: 24887.60 Da
• Isoelectric Point: 7.5391

>T64942 WP_059642481.1 NZ_CP016636:3954383-3955027 [Burkholderia pseudomallei]
MLYTSLNPEKALIWRIVHRDNLPWILDNGLYCGNSAVKAPAWVNIGNPELIDKRAHHPVPLPPRGLLNDYVPFYFTPFSP
MLRNIHTGWGGIQKRPNEEIVILVSSLHHIAGLGLPFLFTDSHAYYRWADYYSDLAYLDKIDWPLLQRRDFKRDPDDPAK
FERYQAEALIHQHLPVGGLLGIVCYTEPLKQRIEQEIQARNLNLPVYARTGWYF

Nucleotide

• Length: 645 bp

>T64942 NZ_CP016636:3954383-3955027 [Burkholderia pseudomallei]
ATGCTCTATACCAGCCTTAATCCGGAAAAAGCACTGATCTGGCGGATTGTCCACCGCGACAATCTGCCGTGGATTCTGGA
CAATGGCCTGTATTGCGGCAACAGCGCGGTGAAGGCGCCGGCATGGGTGAATATCGGTAACCCGGAGCTTATCGACAAGC
GCGCCCATCACCCGGTGCCGTTGCCGCCGCGCGGCTTGCTGAATGACTATGTACCGTTCTATTTCACGCCCTTCTCGCCG
ATGCTGCGTAACATTCACACCGGATGGGGCGGAATCCAGAAGCGGCCGAATGAAGAAATCGTGATCTTGGTGTCTAGCCT
GCACCACATTGCAGGGTTGGGCTTGCCGTTTTTGTTTACTGACAGTCATGCCTATTATCGTTGGGCAGATTACTACTCGG
ACTTGGCCTACCTCGATAAGATCGACTGGCCTTTGCTACAGCGAAGGGATTTCAAGCGTGACCCGGACGACCCCGCCAAG
TTCGAGCGCTATCAGGCGGAGGCGCTGATTCACCAACACTTGCCAGTGGGCGGACTTCTCGGCATCGTGTGCTATACGGA
ACCTTTGAAACAACGCATCGAACAGGAGATTCAGGCCCGTAATCTGAACCTGCCGGTTTATGCCCGAACAGGGTGGTACT
TCTGA

Antitoxin

Protein

• Length: 359 a.a.
• Molecular weight: 40560.52 Da
• Isoelectric Point: 6.0092

>AT64942 WP_065793620.1 NZ_CP016636:3955027-3956103 [Burkholderia pseudomallei]
MITFTQGNLLEARTEALVNTVNTVGVMGKGIALMFKERFDENFRRYAAACKAKEVQTGKMFVTPVHELDGPRWIVNFPTK
QHWRAPSRMEWVVEGLQDLRRFLIEQQVQSIAIPPLGAGNGGLEWADVREQIELALGDLDIDIVVFEPTKQYQNVAKRAG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERAIERFNPGENPLNLQFAPHKYGPYANRLDHLLNNLDGSYLH
SEKRIGDANPLDVIWFDDERKAFVQTYLKSEAKAYTQALEFTAALIDGFESPFGMELLATVDWLLTREGVAPNVPALREG
LRHWRGGSDAATRKDRLFDDRALGIALERLTQPATALV

Nucleotide

• Length: 1077 bp

>AT64942 NZ_CP016636:3955027-3956103 [Burkholderia pseudomallei]
ATGATTACATTCACTCAAGGCAATTTGTTGGAGGCCCGCACTGAGGCGCTGGTCAACACCGTCAACACCGTCGGGGTGAT
GGGCAAGGGCATCGCGCTGATGTTCAAGGAACGCTTTGATGAGAACTTCCGCCGCTATGCCGCCGCCTGCAAAGCGAAGG
AAGTGCAGACAGGCAAGATGTTTGTGACGCCGGTGCACGAACTCGATGGGCCGCGCTGGATCGTTAATTTCCCTACCAAG
CAGCACTGGCGTGCGCCCTCGCGCATGGAGTGGGTGGTGGAAGGCTTGCAGGACCTGCGGCGCTTCTTGATCGAGCAACA
GGTTCAATCCATCGCTATCCCGCCGCTGGGTGCGGGTAACGGTGGCCTGGAATGGGCTGATGTGCGTGAGCAGATCGAAC
TGGCGTTGGGCGATCTGGACATCGACATTGTGGTGTTCGAGCCCACCAAGCAATACCAGAACGTGGCCAAGCGTGCTGGC
GTAGAAAAGCTGACGCCCGCCCGAGCGCTGATCGCCGAATTGGTGCGCCGGTACTGGGTGCTGGGCATGGAATGCAGCTT
GCTGGAGATCCAGAAGCTGGCGTGGTTTCTGGAACGCGCCATTGAGCGTTTCAACCCAGGTGAAAACCCGCTGAACTTGC
AGTTTGCACCACACAAATACGGTCCCTACGCCAACCGACTTGACCACCTGTTGAACAATCTGGATGGCAGCTACCTGCAC
TCTGAAAAGCGCATCGGCGATGCCAATCCGCTGGACGTGATTTGGTTCGACGACGAACGCAAGGCTTTTGTTCAGACATA
TCTCAAGAGCGAAGCCAAGGCCTACACGCAAGCTCTTGAATTCACGGCGGCACTGATCGATGGCTTTGAATCACCCTTTG
GCATGGAACTGCTGGCGACCGTGGATTGGTTGCTGACCCGTGAAGGCGTGGCACCCAACGTGCCGGCCTTGCGCGAAGGG
CTCCGTCATTGGCGCGGTGGTTCAGATGCTGCCACCCGTAAGGACCGCCTTTTTGATGATCGGGCACTGGGCATCGCCCT
GGAGCGTCTGACACAGCCTGCGACAGCGCTGGTGTGA