64941

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 4842130..4843859 Replicon chromosome
Accession NZ_CP016634
Organism Pseudomonas putida strain IEC33019

Toxin (Protein)


Gene name pmenT Uniprot ID A0A1B2FCG6
Locus tag IEC33019_RS22615 Protein ID WP_070091998.1
Coordinates 4843218..4843859 (-) Length 214 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A1B2FCH1
Locus tag IEC33019_RS22610 Protein ID WP_070091997.1
Coordinates 4842130..4843218 (-) Length 363 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEC33019_RS22595 4838027..4838563 + 537 WP_070091994.1 type 1 glutamine amidotransferase -
IEC33019_RS22600 4838755..4839951 + 1197 WP_070091995.1 class I SAM-dependent rRNA methyltransferase -
IEC33019_RS22605 4840227..4842068 + 1842 WP_070091996.1 dihydroxy-acid dehydratase -
IEC33019_RS22610 4842130..4843218 - 1089 WP_070091997.1 macro domain-containing protein Antitoxin
IEC33019_RS22615 4843218..4843859 - 642 WP_070091998.1 DUF4433 domain-containing protein Toxin
IEC33019_RS22620 4843856..4844437 - 582 WP_070091999.1 DUF4433 domain-containing protein -
IEC33019_RS22625 4844526..4845581 - 1056 WP_070092000.1 phosphorylcholine phosphatase -
IEC33019_RS22630 4845828..4847219 + 1392 WP_070092001.1 L-cystine transporter -
IEC33019_RS22635 4847325..4847840 - 516 WP_070092002.1 dihydrofolate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(14-213)

Antitoxin

Macro domain

(18-130)


Sequences

Toxin        


Download         Length: 214 a.a.        Molecular weight: 24558.06 Da        Isoelectric Point: 7.9130

>T64941 WP_070091998.1 NZ_CP016634:c4843859-4843218 [Pseudomonas putida]
MTYTRLNPSKALIWRIVHRDNLPWLLDNGVCAVSTGTLSSGYVGIGNPDLISKRAKRGVPIDPGGTLADYVPFYFTPFSV
MMKNIHSGRGVQQRRNEEIVILVSSLYHVRSLGLPFVFTDAHAYPDWAGYYSDLEHLDKIDWEILQSRDFKRDPDDPRKM
ERYQAEALIYEHVPVAGLLGIVCYNEALQRRVDQEVRQRRLDLPVYARPGWYF

Download         Length: 642 bp

>T64941 NZ_CP016634:c4843859-4843218 [Pseudomonas putida]
ATGACCTATACCAGACTGAACCCAAGCAAAGCCCTGATATGGCGAATCGTTCATCGCGATAACCTTCCGTGGCTTCTGGA
CAACGGCGTATGTGCTGTCTCCACAGGAACGCTATCCAGTGGGTACGTAGGGATTGGCAACCCTGACCTGATTAGCAAGC
GAGCCAAGCGTGGGGTTCCCATCGACCCCGGCGGAACCTTGGCAGACTATGTGCCGTTTTACTTCACACCGTTTTCAGTG
ATGATGAAAAACATTCACTCAGGACGAGGAGTACAGCAACGTAGGAACGAGGAGATCGTGATTCTTGTCTCGAGTCTGTA
CCATGTGCGGTCTCTCGGCCTACCTTTTGTCTTTACCGATGCTCATGCCTATCCGGATTGGGCTGGCTATTACAGCGACC
TAGAGCATCTGGACAAGATTGATTGGGAGATCCTGCAGTCCAGAGATTTCAAGCGTGATCCCGATGATCCGCGGAAAATG
GAACGCTATCAGGCTGAAGCACTGATCTACGAACACGTTCCAGTCGCTGGCCTTCTAGGGATTGTCTGCTACAACGAAGC
TTTGCAACGTCGTGTTGATCAGGAGGTCCGACAACGTCGGTTGGACCTTCCTGTATACGCTCGACCCGGGTGGTATTTCT
GA

Antitoxin


Download         Length: 363 a.a.        Molecular weight: 40557.32 Da        Isoelectric Point: 5.4469

>AT64941 WP_070091997.1 NZ_CP016634:c4843218-4842130 [Pseudomonas putida]
MIRFTKGNLLEAKAEALVNTVNTVGVMGKGIALMFKERFAENYRLYSAACKADEVVIGKMFVTEVRELEGPRWVVNFPTK
RHWRSSSQLAWITDGLHDLRDFILTNEVNSIAIPPLGAGNGGLPWAAVREQIELVLGGLNADVLVFEPTSKYQNVSKRTG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERSIEHFTPDNNPLNLQFSAHKYGPYTNRLEHLLNNLDGSYLH
CDKRIGDAGPLDVIWFDEGRKAFVDTYLHSEAKPYLPALEFTTELIDGFESPFGMELLATVDWLLHQDGVEPTVDAVREG
VKNWSGGQEAAARKSGLFDDDSLATALNRLLIFQAQGITPTH

Download         Length: 1089 bp

>AT64941 NZ_CP016634:c4843218-4842130 [Pseudomonas putida]
ATGATTCGCTTTACGAAAGGTAACCTGCTTGAGGCCAAGGCCGAAGCGCTGGTTAACACAGTCAACACCGTTGGAGTGAT
GGGAAAAGGTATTGCGCTGATGTTCAAGGAGCGCTTTGCTGAAAACTATCGCCTGTACTCGGCTGCCTGCAAAGCGGATG
AAGTCGTCATTGGAAAGATGTTCGTCACTGAGGTTCGTGAACTGGAGGGACCGCGTTGGGTCGTGAATTTTCCGACCAAG
CGTCATTGGCGCTCATCCTCGCAGCTTGCCTGGATCACCGATGGATTGCATGACCTACGGGATTTCATTCTTACCAACGA
AGTAAACTCAATCGCAATTCCTCCATTGGGTGCGGGAAATGGGGGTTTACCTTGGGCTGCTGTGCGTGAACAGATTGAAC
TGGTTTTGGGAGGGCTGAATGCTGACGTCCTGGTTTTCGAGCCCACGAGCAAGTATCAGAATGTTTCTAAACGAACTGGT
GTCGAGAAACTTACCCCTGCCCGAGCACTCATAGCCGAGTTGGTGCGCCGTTATTGGGTACTTGGTATGGAGTGCAGCTT
GCTGGAGATCCAGAAGCTGGCTTGGTTCCTGGAGCGTTCAATCGAGCATTTCACACCTGATAACAACCCGCTTAACCTAC
AGTTTAGTGCTCACAAATACGGGCCTTACACTAACCGATTAGAGCATCTACTGAATAATCTTGACGGCAGTTATCTTCAT
TGTGACAAGCGTATCGGTGATGCGGGTCCACTTGATGTGATCTGGTTCGATGAGGGGCGTAAGGCATTTGTGGATACTTA
CTTGCACAGTGAAGCTAAACCCTATTTGCCAGCCTTGGAGTTCACTACTGAGCTTATTGATGGCTTTGAGTCGCCGTTTG
GCATGGAACTTCTGGCAACGGTCGACTGGCTACTTCATCAGGATGGTGTGGAACCGACGGTCGATGCTGTTCGCGAAGGT
GTGAAGAATTGGTCAGGTGGTCAGGAGGCCGCTGCTCGCAAGAGTGGCTTATTTGATGATGATTCTTTGGCAACTGCGCT
TAACAGGCTCTTGATTTTTCAAGCTCAAGGCATCACGCCAACACATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B2FCG6

Antitoxin

Source ID Structure
AlphaFold DB A0A1B2FCH1

References