Detailed information of TA system TA064940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-DUF971
• Location: 3604669..3605257
• Replicon: chromosome
• Accession: NZ_CP016634
• Organism: Pseudomonas putida strain IEC33019

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: IEC33019_RS16895
• Protein ID: WP_081337477.1
• Coordinates: 3604669..3604971 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A1B2F919
• Locus tag: IEC33019_RS16900
• Protein ID: WP_070093231.1
• Coordinates: 3604964..3605257 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IEC33019_RS16875	3600903..3601796	-	894	WP_070093227.1	LysR family transcriptional regulator	-
IEC33019_RS16880	3601902..3603074	+	1173	WP_070093228.1	YbfB/YjiJ family MFS transporter	-
IEC33019_RS16885	3603100..3604026	-	927	WP_070093229.1	DMT family transporter	-
IEC33019_RS16890	3604133..3604525	-	393	WP_070093230.1	transcriptional regulator	-
IEC33019_RS16895	3604669..3604971	+	303	WP_081337477.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IEC33019_RS16900	3604964..3605257	+	294	WP_070093231.1	putative addiction module antidote protein	Antitoxin
IEC33019_RS16905	3605372..3605644	+	273	WP_070093232.1	hypothetical protein	-
IEC33019_RS16910	3605775..3607100	-	1326	WP_070093233.1	DEAD/DEAH box helicase	-
IEC33019_RS16915	3607245..3608537	+	1293	WP_070093234.1	mechanosensitive ion channel family protein	-
IEC33019_RS16920	3608581..3609462	-	882	WP_070093235.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3596866..3614950	18084

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (6-94)

Antitoxin

• Uncharacterized protein HI1420 (11-93)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11495.24 Da
• Isoelectric Point: 10.2781

>T64940 WP_081337477.1 NZ_CP016634:3604669-3604971 [Pseudomonas putida]
MNKIESSTFRHWLNNLKDEIGKARIVARINRLIEGLPGDISPIGHGVSELRIHYGPGYRVYFHQMGDTLLILLCAGDKGS
QRRDIKTAHQILRSWRMQND

Nucleotide

• Length: 303 bp

>T64940 NZ_CP016634:3604669-3604971 [Pseudomonas putida]
ATGAATAAAATTGAATCAAGCACCTTCAGACATTGGCTGAATAACCTCAAGGATGAAATTGGAAAAGCTCGCATCGTTGC
GCGCATCAACCGGCTGATCGAAGGCTTACCCGGCGATATCTCGCCGATAGGACACGGCGTGAGCGAACTCCGTATCCATT
ACGGACCTGGTTATCGCGTGTACTTTCATCAAATGGGCGATACGTTACTGATTCTGCTGTGCGCAGGCGATAAGGGCAGC
CAACGTCGCGATATCAAGACGGCCCATCAAATCTTACGGTCGTGGAGGATGCAAAATGACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10561.98 Da
• Isoelectric Point: 5.0747

>AT64940 WP_070093231.1 NZ_CP016634:3604964-3605257 [Pseudomonas putida]
MTETFHEFDPADYLTTPEAIAAFMTDALETGDASYVAKAVGVVARAKGMTELARETGLSREQLYRSFSEHGNPTLKSFLT
VMKALGIDMSARPHPPP

Nucleotide

• Length: 294 bp

>AT64940 NZ_CP016634:3604964-3605257 [Pseudomonas putida]
ATGACTGAAACGTTCCATGAATTCGATCCTGCGGACTACCTCACGACCCCCGAGGCCATTGCAGCCTTCATGACAGATGC
CCTCGAAACTGGAGATGCCAGCTACGTCGCCAAAGCCGTCGGTGTCGTGGCTCGCGCCAAAGGCATGACTGAACTGGCGA
GAGAAACAGGGCTATCTCGTGAGCAGCTGTACCGCTCCTTCAGCGAACACGGCAATCCCACCCTCAAGTCGTTCCTGACC
GTCATGAAGGCGCTCGGCATCGACATGAGTGCACGCCCCCATCCGCCGCCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1B2F919