Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2508347..2508985 Replicon chromosome
Accession NZ_CP016628
Organism Escherichia coli strain FORC_041

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag FORC41_RS12990 Protein ID WP_000813794.1
Coordinates 2508809..2508985 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FORC41_RS12985 Protein ID WP_001270286.1
Coordinates 2508347..2508763 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC41_RS12965 2503499..2504440 - 942 WP_001251319.1 ABC transporter permease -
FORC41_RS12970 2504441..2505454 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
FORC41_RS12975 2505472..2506617 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
FORC41_RS12980 2506859..2508268 - 1410 WP_047081861.1 PLP-dependent aminotransferase family protein -
FORC41_RS12985 2508347..2508763 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FORC41_RS12990 2508809..2508985 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FORC41_RS12995 2509207..2509437 + 231 WP_000494244.1 YncJ family protein -
FORC41_RS13000 2509529..2511490 - 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FORC41_RS13005 2511563..2512099 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
FORC41_RS13010 2512152..2513366 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2513406..2514554 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T64934 WP_000813794.1 NZ_CP016628:c2508985-2508809 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T64934 NZ_CP016628:c2508985-2508809 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT64934 WP_001270286.1 NZ_CP016628:c2508763-2508347 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT64934 NZ_CP016628:c2508763-2508347 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References