Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1397957..1398582 Replicon chromosome
Accession NZ_CP016628
Organism Escherichia coli strain FORC_041

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag FORC41_RS07260 Protein ID WP_000911329.1
Coordinates 1398184..1398582 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag FORC41_RS07255 Protein ID WP_000450524.1
Coordinates 1397957..1398184 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC41_RS07225 1393759..1394229 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
FORC41_RS07230 1394229..1394801 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
FORC41_RS07235 1394947..1395825 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
FORC41_RS07240 1395842..1396876 + 1035 WP_047081704.1 outer membrane protein assembly factor BamC -
FORC41_RS07250 1397089..1397802 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
FORC41_RS07255 1397957..1398184 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
FORC41_RS07260 1398184..1398582 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
FORC41_RS07265 1398729..1399592 + 864 WP_047081705.1 neutral zinc metallopeptidase -
FORC41_RS07270 1399607..1401622 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
FORC41_RS07275 1401696..1402394 + 699 WP_000679823.1 esterase -
FORC41_RS07280 1402504..1402704 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T64923 WP_000911329.1 NZ_CP016628:1398184-1398582 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T64923 NZ_CP016628:1398184-1398582 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT64923 WP_000450524.1 NZ_CP016628:1397957-1398184 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT64923 NZ_CP016628:1397957-1398184 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References