Detailed information of TA system TA064916

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 60910..61469
• Replicon: plasmid p0157
• Accession: NZ_CP016626
• Organism: Escherichia coli O157:H7 strain FRIK944

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: A9L45_RS30195
• Protein ID: WP_000421248.1
• Coordinates: 61194..61469 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: A9L45_RS30190
• Protein ID: WP_001178089.1
• Coordinates: 60910..61194 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9L45_RS30150	56102..56662	+	561	WP_000840472.1	fertility inhibition protein FinO	-
A9L45_RS30155	56795..57007	+	213	WP_001302189.1	ANR family transcriptional regulator	-
A9L45_RS30160	57252..57713	+	462	WP_001233853.1	thermonuclease family protein	-
A9L45_RS30165	57759..57968	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
A9L45_RS30170	58006..58344	+	339	WP_000766796.1	hypothetical protein	-
A9L45_RS30175	58584..58838	+	255	WP_000083831.1	replication regulatory protein RepA	-
A9L45_RS30180	59074..59148	+	75	WP_001370046.1	RepA leader peptide Tap	-
A9L45_RS30185	59141..59998	+	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
A9L45_RS30190	60910..61194	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
A9L45_RS30195	61194..61469	+	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A9L45_RS30200	61564..61770	+	207	WP_001105064.1	hypothetical protein	-
A9L45_RS30205	61870..62085	-	216	Protein_68	transposase	-
A9L45_RS33040	62129..62695	-	567	Protein_69	IS3-like element IS600 family transposase	-
A9L45_RS30215	62695..63111	-	417	Protein_70	IS91 family transposase	-
A9L45_RS30220	63111..63296	-	186	WP_001302179.1	hypothetical protein	-
A9L45_RS30225	63473..65683	+	2211	WP_000592771.1	catalase/peroxidase KatP	-
A9L45_RS30230	65727..66116	+	390	WP_001172748.1	cytochrome b562 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyD / toxB / espP / stcE / exeE / exeG	1..95598	95598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T64916 WP_000421248.1 NZ_CP016626:61194-61469 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T64916 NZ_CP016626:61194-61469 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT64916 WP_001178089.1 NZ_CP016626:60910-61194 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT64916 NZ_CP016626:60910-61194 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8