Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 14830..15355 Replicon plasmid p0157
Accession NZ_CP016626
Organism Escherichia coli O157:H7 strain FRIK944

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag A9L45_RS29940 Protein ID WP_001159868.1
Coordinates 15050..15355 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag A9L45_RS29935 Protein ID WP_000813634.1
Coordinates 14830..15048 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9L45_RS34115 10113..10343 + 231 WP_010891288.1 hypothetical protein -
A9L45_RS29900 10464..11204 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
A9L45_RS29905 11489..12466 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
A9L45_RS29915 12874..13074 - 201 WP_000708307.1 hypothetical protein -
A9L45_RS29920 13071..13691 - 621 WP_001248529.1 hypothetical protein -
A9L45_RS29925 13688..14371 - 684 WP_010891291.1 DNA-binding protein -
A9L45_RS29935 14830..15048 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A9L45_RS29940 15050..15355 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
A9L45_RS29945 15356..16162 + 807 WP_000016989.1 site-specific integrase -
A9L45_RS29960 18174..18929 + 756 WP_000852148.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyC / hlyA / hlyB / hlyD / toxB / espP / stcE / exeE / exeG 1..95598 95598


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T64915 WP_001159868.1 NZ_CP016626:15050-15355 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T64915 NZ_CP016626:15050-15355 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT64915 WP_000813634.1 NZ_CP016626:14830-15048 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT64915 NZ_CP016626:14830-15048 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References