Detailed information of TA system TA064888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2322142..2322613
• Replicon: chromosome
• Accession: NZ_CP016625
• Organism: Escherichia coli O157:H7 strain FRIK944

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: A9L45_RS12100
• Protein ID: WP_001303511.1
• Coordinates: 2322335..2322613 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: A9L45_RS12095
• Protein ID: WP_001302048.1
• Coordinates: 2322142..2322333 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9L45_RS12050	2317349..2317933	-	585	WP_000206793.1	DUF551 domain-containing protein	-
A9L45_RS12055	2317990..2318385	-	396	WP_001118159.1	DUF977 family protein	-
A9L45_RS12060	2318401..2319170	-	770	Protein_2308	DUF1627 domain-containing protein	-
A9L45_RS12070	2319196..2319936	-	741	WP_000788936.1	ATP-binding protein	-
A9L45_RS12075	2319943..2320905	-	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
A9L45_RS12080	2320928..2321353	-	426	WP_000693943.1	Rha family transcriptional regulator	-
A9L45_RS12085	2321350..2321652	-	303	WP_000172738.1	transcriptional regulator	-
A9L45_RS33560	2321750..2322121	+	372	WP_001169686.1	hypothetical protein	-
A9L45_RS12095	2322142..2322333	+	192	WP_001302048.1	hypothetical protein	Antitoxin
A9L45_RS12100	2322335..2322613	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A9L45_RS12110	2322966..2323265	+	300	WP_001240334.1	hypothetical protein	-
A9L45_RS12115	2323337..2323555	+	219	WP_001171930.1	hypothetical protein	-
A9L45_RS31485	2324124..2324312	+	189	WP_000449192.1	cell division inhibition protein DicB	-
A9L45_RS12120	2324309..2324500	+	192	WP_001090200.1	DUF1482 family protein	-
A9L45_RS12125	2324593..2327064	+	2472	WP_000048458.1	exonuclease	-
A9L45_RS12130	2327137..2327388	+	252	WP_000005552.1	excisionase family protein	-
A9L45_RS34690	2327423..2327607	+	185	Protein_2322	DUF3596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ospG	2177165..2337493	160328

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T64888 WP_001303511.1 NZ_CP016625:2322335-2322613 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T64888 NZ_CP016625:2322335-2322613 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT64888 WP_001302048.1 NZ_CP016625:2322142-2322333 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT64888 NZ_CP016625:2322142-2322333 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0