Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2020964..2021602 Replicon chromosome
Accession NZ_CP016625
Organism Escherichia coli O157:H7 strain FRIK944

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag A9L45_RS10415 Protein ID WP_000813794.1
Coordinates 2021426..2021602 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag A9L45_RS10410 Protein ID WP_001270286.1
Coordinates 2020964..2021380 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9L45_RS10390 2016116..2017057 - 942 WP_001251315.1 ABC transporter permease -
A9L45_RS10395 2017058..2018071 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
A9L45_RS10400 2018089..2019234 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
A9L45_RS10405 2019479..2020885 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
A9L45_RS10410 2020964..2021380 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
A9L45_RS10415 2021426..2021602 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
A9L45_RS10420 2021824..2022054 + 231 WP_000494244.1 YncJ family protein -
A9L45_RS10425 2022146..2024107 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
A9L45_RS10430 2024180..2024716 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
A9L45_RS10435 2024769..2025980 + 1212 WP_071525382.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T64883 WP_000813794.1 NZ_CP016625:c2021602-2021426 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T64883 NZ_CP016625:c2021602-2021426 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT64883 WP_001270286.1 NZ_CP016625:c2021380-2020964 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT64883 NZ_CP016625:c2021380-2020964 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References