Detailed information of TA system TA064872

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1185805..1186600
• Replicon: chromosome
• Accession: NZ_CP016625
• Organism: Escherichia coli O157:H7 strain FRIK944

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: A9L45_RS05815
• Protein ID: WP_000854914.1
• Coordinates: 1186226..1186600 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9LM11
• Locus tag: A9L45_RS05810
• Protein ID: WP_001280953.1
• Coordinates: 1185805..1186179 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9L45_RS05770	1181160..1182065	+	906	WP_000203541.1	chemotaxis protein	-
A9L45_RS05775	1182062..1183132	+	1071	WP_000102669.1	patatin-like phospholipase family protein	-
A9L45_RS05785	1183472..1184290	+	819	WP_001234682.1	DUF945 domain-containing protein	-
A9L45_RS05790	1184381..1184866	+	486	WP_000214398.1	antirestriction protein	-
A9L45_RS05795	1184882..1185358	+	477	WP_001186738.1	RadC family protein	-
A9L45_RS05800	1185421..1185642	+	222	WP_000692323.1	DUF987 domain-containing protein	-
A9L45_RS05810	1185805..1186179	+	375	WP_001280953.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
A9L45_RS05815	1186226..1186600	+	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
A9L45_RS05820	1186597..1187088	+	492	WP_000976857.1	hypothetical protein	-
A9L45_RS05825	1187100..1187297	+	198	WP_000839282.1	DUF957 domain-containing protein	-
A9L45_RS05830	1187382..1188224	+	843	WP_001280481.1	DUF4942 domain-containing protein	-
A9L45_RS05840	1188715..1188933	-	219	WP_001040187.1	translation initiation factor IF-1	-
A9L45_RS05845	1189218..1189922	-	705	WP_001241680.1	leucyl/phenylalanyl-tRNA--protein transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T64872 WP_000854914.1 NZ_CP016625:1186226-1186600 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T64872 NZ_CP016625:1186226-1186600 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13734.56 Da
• Isoelectric Point: 6.6249

>AT64872 WP_001280953.1 NZ_CP016625:1185805-1186179 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPALATTS

Nucleotide

• Length: 375 bp

>AT64872 NZ_CP016625:1185805-1186179 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACTCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5

Antitoxin

• AlphaFold DB: A0A7U9LM11