Detailed information of TA system TA064864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 159723..160344
• Replicon: plasmid unnamed3
• Accession: NZ_CP016618
• Organism: Microvirga ossetica strain V5/3m

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BB934_RS35275
• Protein ID: WP_099514454.1
• Coordinates: 159943..160344 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BB934_RS35270
• Protein ID: WP_099514453.1
• Coordinates: 159723..159953 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BB934_RS35245	155046..155651	+	606	WP_099514450.1	NAD(P)H-binding protein	-
BB934_RS35250	155686..156231	+	546	WP_099514853.1	flavodoxin	-
BB934_RS35255	156228..157076	+	849	WP_099514451.1	formylglycine-generating enzyme family protein	-
BB934_RS35260	157197..158378	+	1182	WP_099514452.1	adenylate/guanylate cyclase domain-containing protein	-
BB934_RS35265	158589..159218	-	630	WP_099514854.1	NAD-dependent dehydratase	-
BB934_RS35270	159723..159953	+	231	WP_099514453.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BB934_RS35275	159943..160344	+	402	WP_099514454.1	PIN domain-containing protein	Toxin
BB934_RS35280	160402..161318	+	917	Protein_161	recombinase family protein	-
BB934_RS35290	162213..163109	-	897	Protein_162	aminoglycoside phosphotransferase family protein	-
BB934_RS35295	163276..164232	-	957	WP_099514456.1	IS630 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..712299	712299

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• SpoVT-AbrB domain (5-36)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14457.55 Da
• Isoelectric Point: 4.4493

>T64864 WP_099514454.1 NZ_CP016618:159943-160344 [Microvirga ossetica]
MSGKAFFDTNVVLYLLSEDAAKADRAEALLALGGTVSVQVLNEAVSVMTRKLKMDLDEVREVLAGVRHFCAVEPITTDTH
DVALNVMKRYGFSIYDSLIVAAAVSAGCETLFSEDLQDGQRVQDSLTIVNPFK

Nucleotide

• Length: 402 bp

>T64864 NZ_CP016618:159943-160344 [Microvirga ossetica]
ATGAGCGGTAAGGCCTTTTTCGACACCAATGTCGTCTTGTATCTGCTATCTGAGGATGCCGCCAAAGCTGATCGGGCCGA
GGCTCTCCTGGCTTTGGGGGGCACAGTAAGTGTTCAAGTCTTGAACGAGGCCGTGAGCGTCATGACTCGAAAGCTCAAGA
TGGATCTCGACGAGGTCCGGGAGGTCTTGGCTGGAGTACGGCACTTTTGCGCGGTCGAGCCGATAACCACCGATACCCAT
GATGTGGCGCTCAACGTCATGAAGCGCTACGGCTTCTCGATCTATGACAGCCTAATTGTCGCCGCAGCCGTCAGCGCCGG
CTGCGAGACCCTCTTTTCGGAGGACCTACAAGACGGCCAGCGGGTTCAAGACAGTCTGACCATCGTAAATCCGTTCAAGT
AA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8411.68 Da
• Isoelectric Point: 10.5847

>AT64864 WP_099514453.1 NZ_CP016618:159723-159953 [Microvirga ossetica]
MQVAKWGNSLAVRLPSAVVDALKLKEGDDIEIIVAGSRSFEVSKAPDAAETLARLRRFRGRLPAGFKFDRLEANER

Nucleotide

• Length: 231 bp

>AT64864 NZ_CP016618:159723-159953 [Microvirga ossetica]
ATGCAGGTAGCCAAGTGGGGGAACTCCCTTGCTGTCCGCCTCCCCTCAGCCGTCGTCGACGCGTTGAAGCTGAAGGAAGG
CGACGACATCGAAATCATCGTTGCTGGATCGCGCTCCTTCGAGGTCTCAAAAGCCCCTGATGCGGCCGAGACCCTCGCAC
GCCTGCGGCGATTCCGTGGCCGCCTCCCCGCTGGGTTCAAGTTCGATCGGCTTGAGGCCAATGAGCGGTAA