Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 3340160..3340793 Replicon chromosome
Accession NZ_CP016612
Organism Ralstonia solanacearum FJAT-91

Toxin (Protein)


Gene name vapC Uniprot ID M4UIT8
Locus tag BC427_RS15705 Protein ID WP_016725172.1
Coordinates 3340160..3340573 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8XUX7
Locus tag BC427_RS15710 Protein ID WP_011002949.1
Coordinates 3340560..3340793 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BC427_RS15685 3335538..3337274 + 1737 WP_016725176.1 ABC transporter substrate-binding protein -
BC427_RS15690 3337436..3338932 + 1497 WP_016725175.1 glycerol kinase GlpK -
BC427_RS15695 3338997..3339368 - 372 WP_016725174.1 hypothetical protein -
BC427_RS15700 3339559..3340185 + 627 WP_016725173.1 DUF1415 domain-containing protein -
BC427_RS15705 3340160..3340573 - 414 WP_016725172.1 PIN domain-containing protein Toxin
BC427_RS15710 3340560..3340793 - 234 WP_011002949.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BC427_RS15715 3340850..3342457 - 1608 WP_020832850.1 glucan biosynthesis protein -
BC427_RS15720 3342652..3343902 + 1251 WP_096761512.1 AGE family epimerase/isomerase -
BC427_RS15725 3343910..3345205 + 1296 WP_011002952.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-116)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15087.42 Da        Isoelectric Point: 5.5348

>T64860 WP_016725172.1 NZ_CP016612:c3340573-3340160 [Ralstonia solanacearum FJAT-91]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Download         Length: 414 bp

>T64860 NZ_CP016612:c3340573-3340160 [Ralstonia solanacearum FJAT-91]
ATGCCCGCGACTGAGGCATTCTTCGACAGCAATGTCGTGCTGTACCTGCTGTCGGCCGACACCGCCAAGGCCGATGCGGC
GGAAACGCTGCTGATGACCGGCGGTGTCGTCAGCGTACAGGTACTGAACGAAACGACGCACGTGATGCGCCGCAAGCTGG
CCATGCCGTGGCACGCGATCGAAACGGTGCAGGAGGCCGTGCGTGCGCAATGCCGGGTCGAGCCGCTGACGCTCGAAACC
CATGATCTCGGACGGCGTATCGCAGAACGCTACGGATTGTCGGTCTATGACGCACTGATCGTCGCGGCGGCATTGCTTGC
CGGCTGCAACGTTCTGTATTCCGAGGATATGCAGCACGGCCTGGTGATCGAGCAGCATCTGCGCATCGTCAATCCTTTTC
CGGCACGGGCATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8790.98 Da        Isoelectric Point: 8.7287

>AT64860 WP_011002949.1 NZ_CP016612:c3340793-3340560 [Ralstonia solanacearum FJAT-91]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Download         Length: 234 bp

>AT64860 NZ_CP016612:c3340793-3340560 [Ralstonia solanacearum FJAT-91]
ATGCAAGTCTCGAAATGGGGCAACAGCCTCGCTATCCGGCTGCCGGCGGCCGTTGTGGAGGCGCTTCAGCTGCACGAAGG
CGACGACGTCGAGGTGGTGATCGCGGGCGAGCGCTCGTTTGGCGTCCGCCGCAAGCGCGCTGCGCGCGAGCTTTTCGACC
GGATCCACCAATATCGCGGCAAGCTGCCCGCCGATTTCATCTTCGAGCGCGACCAGGCCAATGCCCGCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M4UIT8

Antitoxin

Source ID Structure
AlphaFold DB A0A223GR76

References