Detailed information of TA system TA064859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1723434..1725029
• Replicon: chromosome
• Accession: NZ_CP016612
• Organism: Ralstonia solanacearum FJAT-91

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: BC427_RS08300
• Protein ID: WP_086706272.1
• Coordinates: 1723434..1724771 (-)
• Length: 446 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0S4UDK3
• Locus tag: BC427_RS08305
• Protein ID: WP_016725279.1
• Coordinates: 1724775..1725029 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BC427_RS08275	1719089..1719829	+	741	WP_011001390.1	protocatechuate 3,4-dioxygenase subunit beta	-
BC427_RS08280	1719826..1720524	+	699	WP_016725453.1	protocatechuate 3,4-dioxygenase subunit alpha	-
BC427_RS08285	1720705..1721268	+	564	WP_080624831.1	RNA 2',3'-cyclic phosphodiesterase	-
BC427_RS08290	1721281..1722009	-	729	WP_021155798.1	DsbA family protein	-
BC427_RS08295	1722235..1723437	-	1203	WP_023470034.1	MFS transporter	-
BC427_RS08300	1723434..1724771	-	1338	WP_086706272.1	type II toxin-antitoxin system HipA family toxin	Toxin
BC427_RS08305	1724775..1725029	-	255	WP_016725279.1	helix-turn-helix transcriptional regulator	Antitoxin
BC427_RS08310	1725133..1725972	-	840	WP_011001397.1	oxaloacetate decarboxylase	-
BC427_RS08320	1726290..1726928	+	639	WP_020831956.1	7-carboxy-7-deazaguanine synthase	-
BC427_RS08325	1726996..1727472	+	477	WP_011001399.1	6-carboxytetrahydropterin synthase QueD	-
BC427_RS08330	1727492..1728043	+	552	WP_016727233.1	tRNA adenosine(34) deaminase TadA	-
BC427_RS08335	1728040..1728966	+	927	WP_016727232.1	muramoyltetrapeptide carboxypeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-107)

Antitoxin

• Transcription regulator, HipB-like (10-66)

Sequences

Toxin

Protein

• Length: 446 a.a.
• Molecular weight: 48754.78 Da
• Isoelectric Point: 6.7232

>T64859 WP_086706272.1 NZ_CP016612:c1724771-1723434 [Ralstonia solanacearum FJAT-91]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDGWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGTSKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Nucleotide

• Length: 1338 bp

>T64859 NZ_CP016612:c1724771-1723434 [Ralstonia solanacearum FJAT-91]
ATGGCGCATCCCGAACTGCATCTGTGGATGAACGGCGAGCATGTCGGCATCTGGACCGCCAGCCGCGCCGGCGTGCCGAT
CCTGCGCTATGACGACGGTTGGCTGCGCTCGCCCAACATGCGGCCGCTGTCGCTGTCGCTGCCGATCCCGGCCGGTGTGC
CCGAGCTGCGCGGCCAGGTGGTCGACGACTACTTCGACAACCTGCTGCCCGAGGCCCAGCCCATCCGCGAGCGCGTGCGG
CGCCGCTTCGGCCTCGCCTCCACAGATGCCGCCGAATTGCTGGCCGCCATCGGCCGCGACTGCGTGGGCGCCGTGCAACT
GCTGCCGCCCGGCATGGCCCCCACCGGCTACGATCGCATCGACAGCGAGCCGCTCACCGACAGCCAGGTCGACACGCTGC
TGCGCAACCTCACCGCCACGCCCGCGCTGGGCGAGGACGCCGGCGACCCCGACGCCTTCCGCATCTCCATTGCCGGCGCG
CAGGAGAAAACCGCCCTGCTGCGCATCGGCGATGCCTGGCACCGGCCGCTGGGCGCCACGCCCACCACGCACATCCTCAA
GCTGCCGCTGGGGCTGATCGGCAACATCCGCGCCGACATGCAGGACTCGGTCGACAACGAATGGCTGTGCGCGCAGCTGC
TGGCGGCCTGGGGGCTGGAGGTGGCGCGCACCGAGATCGCCCGCTTCGGCACCAGCAAAGTGCTGGCGGTCGAGCGCTTC
GACCGTCGCTGGATGCCGGGTAACGCCTGGATCGCGCGCCTGCCGCAGGAAGACTTCTGCCAGGCCACCGGCACGCCCGC
GCACCGCAAATACGAAAATGACGGCGGCCCCGGCATGCGCGCCTGCCTCGACCTGCTGTCCGCGTCCGAACACGCCATCG
ACGACAAGCGCCGCTTCGTGCTGGCGCAACTTGCCTTCTGGCTACTGGCCGCCACCGACGGGCACGCGAAGAACTTCTCG
CTGGCGATCGGGCGCGGCGGGCGCTTCCGCATGACGCCGCTGTATGACGTGCTCTCGGTCTGGCCGATCATGGGCGACGG
GCCGAACCAGCTGTCGCCCTATCGCGCGCGGCTGGCGATGGCCCTGCGCGGCAAGAACGCGCATGCGCGGCTGGCCGACA
TCCACGTGCGCCACTGGCAGGCGCTGGCGGCGCAATCCGGCGTGCCGGACGCGTTCGCCGGCATGCAGGCGCTGGTCGAG
CGCGTGCCGGCGGGTCTCGAGCGGGTCGAGGCGATCCTGCCGCCCGACTTCTCGGGCCGGGTCTGGAGCAGCGTGCGCAC
CGGCGTGCTCGCGCAGGCCGAGCGCTTTCTCAACGAACTGGCGTCCGTCACCCCATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 8754.07 Da
• Isoelectric Point: 10.4693

>AT64859 WP_016725279.1 NZ_CP016612:c1725029-1724775 [Ralstonia solanacearum FJAT-91]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAGTPSAA
PADW

Nucleotide

• Length: 255 bp

>AT64859 NZ_CP016612:c1725029-1724775 [Ralstonia solanacearum FJAT-91]
ATGGAGTCCCTGCTCTCCTCGGCGCCGCAGCTGTCGGCGCATCTGAAATCGTTGCGCAAGGCGCGCGGACTCACGCAGGC
CCAGTTGGGCGCACTGCTGGGCGTCAGCCAGAACCGCATCGCCGACATCGAAAAAGACCCGGGCGCGGTCGGCTTCGAGC
AGTTACTGCGCATCCTGCACGCGCTCGGCGCGCAGTTGACGCTGCGCACGTCCGCGCCCGCCGGCACCCCCTCCGCCGCG
CCCGCCGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S4UDK3