Detailed information of TA system TA064858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 1049589..1050250
• Replicon: chromosome
• Accession: NZ_CP016612
• Organism: Ralstonia solanacearum FJAT-91

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A9E7Z845
• Locus tag: BC427_RS05135
• Protein ID: WP_016727753.1
• Coordinates: 1049589..1050002 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A9E7Z6P3
• Locus tag: BC427_RS05140
• Protein ID: WP_019717749.1
• Coordinates: 1049999..1050250 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BC427_RS05105	1044911..1045198	-	288	WP_086706365.1	hypothetical protein	-
BC427_RS05110	1045151..1045714	+	564	Protein_1013	hypothetical protein	-
BC427_RS26320	1045723..1045875	+	153	WP_157698851.1	hypothetical protein	-
BC427_RS05115	1045951..1046595	+	645	WP_086706230.1	hypothetical protein	-
BC427_RS26325	1046829..1047674	-	846	WP_157698852.1	hypothetical protein	-
BC427_RS26330	1048062..1048355	-	294	WP_020371973.1	hypothetical protein	-
BC427_RS05130	1048495..1049517	+	1023	WP_086706232.1	DNA cytosine methyltransferase	-
BC427_RS05135	1049589..1050002	-	414	WP_016727753.1	type II toxin-antitoxin system VapC family toxin	Toxin
BC427_RS05140	1049999..1050250	-	252	WP_019717749.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BC427_RS05145	1050627..1050859	+	233	Protein_1021	hypothetical protein	-
BC427_RS26335	1051013..1051228	-	216	WP_071615547.1	hypothetical protein	-
BC427_RS26775	1051502..1051840	+	339	WP_016727784.1	glycine zipper 2TM domain-containing protein	-
BC427_RS05155	1052515..1053231	-	717	WP_049800424.1	Crp/Fnr family transcriptional regulator	-
BC427_RS26340	1053932..1054546	+	615	WP_157698853.1	hypothetical protein	-
BC427_RS26345	1054607..1054756	-	150	WP_155738951.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1054753..1055718	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-67)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15586.89 Da
• Isoelectric Point: 6.2337

>T64858 WP_016727753.1 NZ_CP016612:c1050002-1049589 [Ralstonia solanacearum FJAT-91]
MSYLIDTNVLSELRRKAPDARVVAWMQDRPRQSLYLSVLTLGEIRKGIERLDDAARRQHLIDWLEVELPNYFLGRLLDID
AHTADRWGRLMSSAGRPLPAIDGLLAATALQHDLTLVTRNTKDFAGLDVQLINPWEA

Nucleotide

• Length: 414 bp

>T64858 NZ_CP016612:c1050002-1049589 [Ralstonia solanacearum FJAT-91]
TTGAGTTACCTGATCGACACCAATGTCCTGTCCGAATTGCGCCGCAAAGCGCCCGATGCGCGCGTGGTGGCCTGGATGCA
GGACCGCCCGCGCCAGTCGCTCTACCTGAGCGTGCTGACCCTGGGCGAGATTCGCAAAGGTATCGAGCGGCTGGACGACG
CGGCGCGCCGCCAGCACCTGATTGACTGGCTCGAAGTGGAACTGCCGAACTACTTTCTCGGCCGATTGCTCGACATCGAT
GCGCATACCGCCGACCGCTGGGGCCGGCTGATGTCCTCGGCCGGCCGCCCCCTGCCCGCCATCGACGGCCTGCTGGCCGC
GACCGCCCTGCAACACGACCTCACGCTGGTCACGCGCAACACCAAGGATTTCGCGGGGCTCGACGTCCAGCTCATCAACC
CATGGGAAGCCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9305.49 Da
• Isoelectric Point: 5.0774

>AT64858 WP_019717749.1 NZ_CP016612:c1050250-1049999 [Ralstonia solanacearum FJAT-91]
MQSWQMQAAKARFSDVVKRAADDGPQEITVHGRPVAVVISRALFDRLSGGGESLVSFMRQSPLADQDDVVFERERSLPRE
VEF

Nucleotide

• Length: 252 bp

>AT64858 NZ_CP016612:c1050250-1049999 [Ralstonia solanacearum FJAT-91]
ATGCAAAGCTGGCAAATGCAGGCCGCCAAGGCGCGGTTTTCCGATGTCGTGAAGCGGGCGGCAGACGATGGCCCGCAGGA
AATCACCGTGCATGGCCGCCCGGTGGCGGTGGTGATCTCGCGCGCACTGTTCGATCGCTTGAGCGGCGGCGGTGAGTCGC
TGGTCAGCTTCATGCGCCAATCCCCGCTGGCCGACCAGGACGATGTCGTCTTTGAGCGCGAGCGCAGCCTGCCGCGCGAG
GTCGAGTTTTGA